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Et1603 14

Manufactured by Huabio
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The ET1603-14 is a laboratory equipment designed for general use in research and analytical applications. It is a compact and versatile device that can perform various tasks essential for laboratory workflows. The core function of the ET1603-14 is to provide a reliable and consistent platform for researchers and analysts to conduct their experiments and analyses. Further details about the intended use or specific capabilities of the equipment are not available.

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Lab products found in correlation

Ab48187, by Abcam (3 mentions) Ab208035, by Abcam (2 mentions) Ab37152, by Abcam (2 mentions) Ab62484, by Abcam (2 mentions) Ab3214, by Abcam (2 mentions) Ab32503, by Abcam (2 mentions) Ab32370, by Abcam (2 mentions) Rt1196, by Huabio (2 mentions) Er1706 34, by Huabio (2 mentions) Er40402, by Huabio (1 mentions) Df6280, by Affinity Biosciences (1 mentions) Ab34710, by Abcam (1 mentions) A00683 1, by Boster Bio (1 mentions) Df7576, by Affinity Biosciences (1 mentions) Ab8580, by Abcam (1 mentions) Sc 11420, by Santa Cruz Biotechnology (1 mentions) Anti acetyl histone h3, by Cell Signaling Technology (1 mentions)

3 protocols using et1603 14

1

Apoptosis-related Protein Detection

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Primary antibodies were as follows: anti‐FABP4 (JM10‐99; HuaBio), anti‐BAX (ab32503; Abcam), anti‐BCL‐2 (ab3214; Abcam), anti‐BCL‐XL (ab32370; Abcam), anti‐cleaved caspase 3 (66470‐2‐Ig; Proteintech Group), anti‐p‐JNK (4668; Cell Signaling Technology), anti‐JNK (ab208035; Abcam), anti‐p‐eIF2α (ET1603‐14; HuaBio), anti‐eIF2α (RT1196; HuaBio), anti‐p‐PERK (sc32577; Santa Cruz), anti‐GRP78 (ER40402; HuaBio), anti‐IRE1α (ab48187; Abcam), anti‐CHOP (2895; Cell Signaling Technology), anti‐ATF4 (11815; Cell Signaling Technology), anti‐ATF6 (ER1706‐34; HuaBio), anti‐XBP1 (ab37152; Abcam) and anti‐caspase 12 (ab62484; Abcam).
Tan Z., Guo F., Huang Z., Xia Z., Liu J., Tao S., Li L., Feng Y., Du X., Ma L, & Fu P. (2019). Pharmacological and genetic inhibition of fatty acid‐binding protein 4 alleviated cisplatin‐induced acute kidney injury. Journal of Cellular and Molecular Medicine, 23(9), 6260-6270.
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2

Western Blot Analysis of Stress Regulators

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Western blot was performed using a previously published protocol [43 (link)]. The primary antibodies used were as follows: anti-p-PERK (DF7576, Affinity), anti-PERK (ER64553, HuaBio), anti-p-IRE-1α (S724) (ab48187, Abcam), anti-IRE1α (A00683-1, Boster), anti-p-eIF-2α(S51) (ET1603-14, HuaBio), anti-eIF-2α (ET7111-34, HuaBio), anti-Col I (ab34710, Abcam), anti-LDHA (DF6280, Affinity), and β-actin (AC026, ABclonal). After supplementation with the primary antibodies, the lung tissues and cells samples were incubated with goat anti-rabbit or anti-mouse secondary antibodies (074-1506/074-1806; Kirkegaard & Perry Laboratories, Gaithersburg, MD, USA) at a concentration of 1:5000 for 1 h. The target bands were visualized using the ECL prime Western blotting detection reagent.
Mao N., Fan Y., Liu W., Yang H., Yang Y., Li Y., Jin F., Li T., Yang X., Gao X., Cai W., Liu H., Xu H., Li S, & Yang F. (2022). Oxamate Attenuates Glycolysis and ER Stress in Silicotic Mice. International Journal of Molecular Sciences, 23(6), 3013.
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3

Molecular Mechanisms of ER Stress Response

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Anti-HDAC6 (sc11420, Santa Cruz), anti-acetyl-histone H3 (9649, Cell Signaling Technology), Anti-H3 (ab8580, Abcam), anti-BAX (ab32503, Abcam), anti-BCL-2 (ab3214, Abcam), anti-BCL-XL (ab32370, Abcam), anti-cleaved caspase 3 (66470-2-Ig, Proteintech Group), anti-phospho-JNK (4668, Cell Signaling Technology), anti-JNK (ab208035, Abcam), anti-phospho-eIF2α (ET1603-14, HuaBio), anti-eIF2α (RT1196, HuaBio), anti-phospho-PERK (sc32577, Santa Cruz), anti-GRP78 (ER40402, HuaBio), anti-IRE1α (ab48187, Abcam), anti-CHOP (2895, Cell Signaling Technology), anti-ATF4 (11815, Cell Signaling Technology), Anti-ATF6 (ER1706-34, HuaBio), anti-XBP1 (ab37152, Abcam), anti-caspase 12 (ab62484, Abcam). Primer sequences was exhibited in Supplementary Material (Supplementary Table S1).
Hao Y., Guo F., Huang Z., Feng Y., Xia Z., Liu J., Li L., Huang R., Lin L., Ma L, & Fu P. (2020). 2-Methylquinazoline derivative 23BB as a highly selective histone deacetylase 6 inhibitor alleviated cisplatin-induced acute kidney injury. Bioscience Reports, 40(1), BSR20191538.
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