1H NMR and 13C NMR spectra were performed on a Bruker DRX NMR spectrometer in CDCl3 (δ = 7.26 ppm) at 298 K as the internal standard. UV-vis and fluorescence spectra were measured on a Shimadzu spectrophotometer, from Japan, (UV-3600) and a HITACHI spectrometer (F-4600, Japan), respectively. TEM of the nanoparticles were measured on equipment (JEOL JEM-2100). Dynamic light scattering (DLS) of DPPA NPs was tested on a particle size analyzer (90 Plus, Brookhaven Instruments, United States). Fluorescence imaging of DPPA NPs in nude mice was recorded on an IVIS spectrum.
Uv 3600
Manufactured by Hitachi
Sourced in Japan
The UV-3600 is a high-performance UV-Vis-NIR spectrophotometer manufactured by Hitachi. It is designed to measure the absorption, transmission, and reflectance of a wide range of samples in the ultraviolet, visible, and near-infrared regions of the electromagnetic spectrum.
Automatically generated - may contain errors
Lab products found in correlation
Escalab 250xi, by Thermo Fisher Scientific (2 mentions)
90 plus, by Brookhaven Instruments (1 mentions)
Drx nmr spectrometer, by Bruker (1 mentions)
F 4600, by Hitachi (1 mentions)
Jem 2100, by JEOL (1 mentions)
X pert pro mpd, by Malvern Panalytical (1 mentions)
Jem 2100f, by JEOL (1 mentions)
Labram hr evolution, by Horiba (1 mentions)
Su8020, by Hitachi (1 mentions)
U 2910, by Hitachi (1 mentions)
Saxs waxs system, by Xenocs (1 mentions)
Nanoir3, by Bruker (1 mentions)
Sta449f3, by Netzsch (1 mentions)
F 7000, by Hitachi (1 mentions)
5 protocols using uv 3600
1
Detailed Characterization of DPPA Nanoparticles
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1H NMR and 13C NMR spectra were performed on a Bruker DRX NMR spectrometer in CDCl3 (δ = 7.26 ppm) at 298 K as the internal standard. UV-vis and fluorescence spectra were measured on a Shimadzu spectrophotometer, from Japan, (UV-3600) and a HITACHI spectrometer (F-4600, Japan), respectively. TEM of the nanoparticles were measured on equipment (JEOL JEM-2100). Dynamic light scattering (DLS) of DPPA NPs was tested on a particle size analyzer (90 Plus, Brookhaven Instruments, United States). Fluorescence imaging of DPPA NPs in nude mice was recorded on an IVIS spectrum.
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1H NMR and 13C NMR spectra were performed on a Bruker DRX NMR spectrometer in CDCl3 (δ = 7.26 ppm) at 298 K as the internal standard. UV-vis and fluorescence spectra were measured on a Shimadzu spectrophotometer, from Japan, (UV-3600) and a HITACHI spectrometer (F-4600, Japan), respectively. TEM of the nanoparticles were measured on equipment (JEOL JEM-2100). Dynamic light scattering (DLS) of DPPA NPs was tested on a particle size analyzer (90 Plus, Brookhaven Instruments, United States). Fluorescence imaging of DPPA NPs in nude mice was recorded on an IVIS spectrum.
2
Spectrophotometric Analysis of Respiratory Complexes
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Livers of E18.5 neonates were quickly homogenized in phosphate buffer (50 mM KH2PO4, pH 7.4) followed by spectrophotometric analysis of isolated respiratory chain complex activities at 37°C using a spectrophotometer (UV-3600; Hitachi). NADH dehydrogenase activity was determined at 340 nm (ε = 6,220 M/cm) after the addition of 0.25 mM NADH, 0.25 mM decylubiquinone, and 1 mM KCN and controlling for rotenone sensitivity. Succinate dehydrogenase activity was measured at 600 nm (ε = 21,000 M/cm) after addition of 40 mM succinate, 35 µM dichlorophenolindophenol, and 1 mM KCN. The succinate cytochrome c reductase activity was followed at 540 nm (ε = 18,000 M/cm) in the presence of 40 mM succinate, 80 µg/ml cytochrome c, and 1 mM sodium azide. The specific II–III activity was defined as the flux difference before and after the addition of 1 µM antimycin A. The cytochrome c oxidase activity was assessed using a classical TMPD/ascorbate assay. In brief, oxygen consumption was assessed in the presence of 0.2 mM TMPD, 1 mM ascorbate, and 0.5 µM antimycin A. After a few minutes of stationary respiration, 2 mM KCN was injected into the chamber. The cytochrome c oxidase activity corresponds to the KCN-sensitive respiration. All chemicals were obtained from Sigma-Aldrich.
3
Comprehensive Materials Characterization
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A scanning electron microscope (SEM, SU8020, Hitachi, Tokyo, Japan), a transmission electron microscope (TEM, JEM-2100F, Jeol, Akishima, Japan), an X-ray diffractometer (X′Pert PRO MPD, PANalytical, Etten Leur, The Netherlands, Cu Kα radiation), a LabRam HR Evolution instrument (Horiba Jobin Yvon, Palaiseau, France, excitation wavelength: 532 nm), an X-ray photoelectron spectrometer (ESCALAB250Xi, Thermo, Waltham, MA, USA, Al Kα monochromatic radiation), and a UV-3600 (Hitachi, Tokyo, Japan) spectrophotometer were used to study the properties of samples.
4
Absorption and NMR Spectroscopy Protocol
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UV/Vis absorption spectra were recorded by using Jasco J‐820, Shimadzu UV‐3600, and Hitachi U‐2910 spectrophotometers (1 cm or 1 mm quartz cells with screw caps were used). NMR spectroscopy was performed by using Bruker Avance III HD 500 and JEOL AL300BX spectrometers. Tetramethylsilane was used as the internal standard. Titration experiments were performed by the addition of stock solution (acid dissolved in chloroform) into the sample by using a microsyringe. All measurements were performed at 25 °C unless otherwise specified.
5
Comprehensive Structural Characterization of Composite Films
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Fourier transform infrared (Nicolet iN10MX), Raman spectroscopy (LabRAM HR Evolution) with 633 nm laser and wide-angle X-ray scattering (Xenocs Xeuss SAXS/WAXS System) measurements were employed for analyzing crystalline structure. X-ray photoelectron spectra (ESCALAB 250Xi) were performed with a monochromated Al Kα source. The absorption and photoluminescence spectra of the films were measured using an ultraviolet-visible-near infrared spectrometer (Shimadzu UV-3600) and a fluorescence spectrometer (Hitachi F-7000), respectively. Atomic force microscopy-infrared spectroscopy (NanoIR3, Bruker) was operated in contact mode, employing gold-coated Si tips (Bruker, spring constant 0.07–0.4 N m−1), and the AFM-IR absorption spectra were obtained with IR laser irradiating at wavelength 1260 cm−1. Differential scanning calorimetry analysis was performed on a Thermal Analysis Instruments Analyzer (STA-449F3, NETZSCH, Germany) from 30 °C to 300 °C at a heating rate of 10 °C min−1 in Ar atmosphere. The degree of crystallinity in the PVDF matrix was calculated from DSC curves according to the equation where ΔHf and ΔHm100 are the melting enthalpy of composite and 100% crystalline PVDF (103.4 J g−1)53 (link), while ϕ represents the mass fraction of fillers, respectively. TEM images were acquired at JEM-2100F operated at 200 kV.
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