Urine samples were collected immediately after rumen liquid samples. Approximately 40 mL of urine was collected via voluntary excretion or manual stimulation and then acidified with sulfuric acid (10% v/v) to minimize volatilization and then frozen for chemical analysis. Before chemical analysis, all samples were thawed and used to estimate N concentration (%) by a N autoanalyzer LECO FP528 based on DUMAS method [17 ]. Urinary urea (GLDH UV, HUMAN, Wiesbaden, Germany) was estimated using a Wiener Metrolab 2300 auto-analyzer (Wiener Lab., Rosario Argentina).
Metrolab 2300 auto analyzer
Manufactured by Wiener Lab
Sourced in Argentina
The Metrolab 2300 auto-analyzer is a laboratory instrument designed for automated clinical chemistry analysis. It performs photometric measurements to quantify the concentration of various analytes in biological samples. The Metrolab 2300 is capable of processing multiple samples simultaneously and provides standardized, reproducible results.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using metrolab 2300 auto analyzer
1
Quantifying Urinary Nitrogen in Ruminants
2
Bovine Plasma Metabolite Analysis
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Blood samples were obtained by venipuncture coccygeal with heparinized tubes from each cow after the afternoon milking on three occasions (weeks 4, 8, and 11 of the trial). Samples were centrifuged at 800× g for 10 min on the day of sampling, and the obtained plasma was aliquoted and frozen at −20 °C in 1.5 mL microtubes for later analysis. At the end of the trial, β-hydroxybutyrate (β-hydroxybutyrate Rambut, Randox®, Crumlin, UK), non-esterified fatty acids (NEFA, ACS-ACOD, Wako®, Neuss, Deutschland), albumin (BCG, Human®, Wiesbaden, Germany), and urea (GLDH, Human®) were determined in plasma in a Metrolab 2300 autoanalyzer (WienerLab®, Rosario, Argentina).
3
Comprehensive Blood Analysis Protocol
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A 10-mL sample of blood from each animal from each experimental period was obtained from the coccygeal vein using 2 vacuum tubes (Becton Dickinson Co.), one containing EDTA (4 mL) and the other with no anticoagulant (9 mL). Each sample was mixed gently and centrifuged at 3,200 × g for 10 min at room temperature. Whole blood was used for determination of Heinz-Ehrlich bodies in blood smears, prepared on average 5 h after sampling, using crystal violet staining and expressed as percent erythrocytes containing one or more inclusion bodies. Plasma was recovered, transferred to plastic vials, and frozen at -20°C until analysis for albumin, γ-glutamyl transferase (GGT), glutamate dehydrogenase (GLDH), nonesterified FA (NEFA), BHB, and aspartate aminotransferase (AST) using a Metrolab 2300 auto-analyzer (Wiener Lab) at 37°C.
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