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5 6 dimethylbenzimidazole dmb

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5,6-dimethylbenzimidazole (DMB) is a chemical compound used in various laboratory applications. It is a crystalline solid with a molecular formula of C10H12N2. DMB serves as a precursor and intermediate in the synthesis of other chemical compounds, but a detailed description of its core function is not available without the risk of making unsubstantiated claims.

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Lab products found in correlation

Vitamin b12, by Merck Group (2 mentions) Lambda 35 uv vis spectrometer, by PerkinElmer (2 mentions) Yeast extract, by BD (2 mentions) Peptone, by BD (2 mentions) Vinyl chloride, by Merck Group (2 mentions) Ethene, by Merck Group (2 mentions) Purine, by Merck Group (2 mentions) 15nh4cl, by Cambridge Isotopes (2 mentions) Dicyanocobinamide cbi, by Merck Group (2 mentions) 5 methylbenzimidazole 5 mebza, by Merck Group (2 mentions) 5 methoxybenzimidazole 5 omebza, by Merck Group (2 mentions) Benzimidazole bza, by Merck Group (2 mentions)

2 protocols using 5 6 dimethylbenzimidazole dmb

1

Biosynthesis and Purification of Cobamides

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Vitamin B12 (≥98%), dicyanocobinamide (Cbi, ≥93%), 5,6-dimethylbenzimidazole (DMB) (99%), 5-methylbenzimidazole (5-MeBza) (98%), 5-methoxybenzimidazole (5-OMeBza) (97%), benzimidazole (Bza) (98%), purine (98%) were purchased from Sigma-Aldrich. 15NH4Cl (99%) was obtained from Cambridge Isotope Laboratories. Yeast extract and peptone were purchased from Becton, Dickinson and Company. Bza-Cba, 5-MeBza-Cba and 5-OMeBza-Cba were prepared via guided cobamide biosynthesis 10 (link). Factor III (5-OHBza-Cba), norpseudo vitamin B12 and phenolic cobamides (i.e., Phenol-Cba and p-Cresol-Cba) were extracted and purified from Methanosarcina barkeri strain Fusaro (DSM 804), Sulfurospirillum multivorans (DSM 12446) and Sporomusa sp. strain KB-1 (16S rRNA gene GenBank accession no. AY780559.1) cells, respectively. Concentrations of purified cobamides were determined at 361 nm with a Lambda 35 UV-Vis spectrometer (PerkinElmer) using a molar extinction coefficient of 28,060 mole−1 cm−1 12 (link). Ethene (≥99.9%) and vinyl chloride (≥99.5%) were purchased from Sigma-Aldrich. All other chemicals used were reagent grade or higher.
Yan J., Bi M., Bourdon A.K., Farmer A.T., Wang P.H., Molenda O., Quaile A., Jiang N., Yang Y., Yin Y., Şimşir B., Campagna S.R., Edwards E.A, & Löffler F.E. (2017). Purinyl-cobamide is a native prosthetic group of reductive dehalogenases. Nature chemical biology, 14(1), 8-14.
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2

Biosynthesis and Purification of Cobamides

Check if the same lab product or an alternative is used in the 5 most similar protocols
Vitamin B12 (≥98%), dicyanocobinamide (Cbi, ≥93%), 5,6-dimethylbenzimidazole (DMB) (99%), 5-methylbenzimidazole (5-MeBza) (98%), 5-methoxybenzimidazole (5-OMeBza) (97%), benzimidazole (Bza) (98%), purine (98%) were purchased from Sigma-Aldrich. 15NH4Cl (99%) was obtained from Cambridge Isotope Laboratories. Yeast extract and peptone were purchased from Becton, Dickinson and Company. Bza-Cba, 5-MeBza-Cba and 5-OMeBza-Cba were prepared via guided cobamide biosynthesis 10 (link). Factor III (5-OHBza-Cba), norpseudo vitamin B12 and phenolic cobamides (i.e., Phenol-Cba and p-Cresol-Cba) were extracted and purified from Methanosarcina barkeri strain Fusaro (DSM 804), Sulfurospirillum multivorans (DSM 12446) and Sporomusa sp. strain KB-1 (16S rRNA gene GenBank accession no. AY780559.1) cells, respectively. Concentrations of purified cobamides were determined at 361 nm with a Lambda 35 UV-Vis spectrometer (PerkinElmer) using a molar extinction coefficient of 28,060 mole−1 cm−1 12 (link). Ethene (≥99.9%) and vinyl chloride (≥99.5%) were purchased from Sigma-Aldrich. All other chemicals used were reagent grade or higher.
Yan J., Bi M., Bourdon A.K., Farmer A.T., Wang P.H., Molenda O., Quaile A., Jiang N., Yang Y., Yin Y., Şimşir B., Campagna S.R., Edwards E.A, & Löffler F.E. (2017). Purinyl-cobamide is a native prosthetic group of reductive dehalogenases. Nature chemical biology, 14(1), 8-14.
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