Gv493 lentiviral shrna vector

Manufactured by Genechem

Sourced in China

The GV493 lentiviral shRNA vector is a tool for gene knockdown experiments. It is designed to deliver short hairpin RNA (shRNA) constructs into target cells using a lentiviral delivery system. The vector enables stable and efficient gene silencing through the RNA interference (RNAi) pathway.

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Lab products found in correlation

Bt 549, by National Collection of Authenticated Cell Cultures (2 mentions) Hcc1937, by National Collection of Authenticated Cell Cultures (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Mda mb 231, by National Collection of Authenticated Cell Cultures (2 mentions) Hs578t, by National Collection of Authenticated Cell Cultures (2 mentions) Sk br 3, by National Collection of Authenticated Cell Cultures (1 mentions) Mcf 7, by National Collection of Authenticated Cell Cultures (1 mentions) Gv115, by Genechem (1 mentions) Gv657 vector, by Genechem (1 mentions)

Spelling variants of this product

Lentiviral vector (82 citations) GV493 (14 citations) ShRNA vectors (13 citations) GV493 vector (8 citations) Lentiviral vector GV493 (4 citations) Lentiviral shRNA vectors (4 citations) Lentiviral shRNAs (3 citations)

2 protocols using gv493 lentiviral shrna vector

Lentiviral Transduction of Breast Cancer Cells

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Human breast cancer cell lines (MDA-MB-231, HCC1937, BT-549, Hs578T, SKBR-3 and MCF-7) were purchased from the Cell Bank of Type Culture Collection of The Chinese Academy of Sciences. All cell lines were cultured in DMEM (HyClone) supplemented with 10% FBS (Gibco) and 1% penicillin and streptomycin solution at 37 °C under 5% CO2 conditions in a humidified incubator. Short hairpin RNA (shRNA) targeting CTPS1 were subcloned into GV115 and GV493 lentiviral shRNA vector (Genechem, Shanghai, China), respectively. For overexpressing YBX1, the construct was generated by subcloning PCR amplified full-length human YBX1 cDNA into the GV657 vector (Genechem, Shanghai, China). The constructed lentiviral vectors were packaged into the viruses in 293 T cells. Then, the harvested and concentrated viruses were added into cells and cultured for 72 h. The target sequences of the shRNA and negative control were as follows:
shCTPS1-1: 5’-ATCTTGTAGCGGATGATTC-3’.
shCTPS1-2: 5’-GAGGATTTGGTGTTCGAGGA-3’.
shCtrl: 5’-TTCTCCGAACGTGTCACGT-3’.

Lin Y., Zhang J., Li Y., Guo W., Chen L., Chen M., Chen X., Zhang W., Jin X., Jiang M., Xiao H., Wang C., Song C, & Fu F. (2022). CTPS1 promotes malignant progression of triple-negative breast cancer with transcriptional activation by YBX1. Journal of Translational Medicine, 20, 17.

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TNBC Cell Line Manipulation and Lentiviral Transduction

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The human TNBC cell lines MDA-MB-231, BT-549, HCC1937, and Hs578T were purchased from the Cell Bank of Type Culture Collection of The Chinese Academy of Sciences. Cell were cultured in Dulbecco's Modified Eagle Medium (DMEM; HyClone, Logan, USA), supplemented with 10% fetal bovine serum (FBS; Gibco, Carlsbad, USA) and 1% penicillin and streptomycin solution and incubated at 37 °C and 5% CO₂. The short hairpin RNA (shRNA) targeting YTHDF3 was subcloned into the GV493 lentiviral shRNA vector (Genechem, Shanghai, China). For overexpression of ZEB1, the construct was generated by subcloning PCR amplified full-length human ZEB1 cDNA into the vector. The constructed lentiviral vectors were packaged into viruses in 293 T cells which were then harvested and the concentrated viruses were added to TNBC cells and cultured for 72 hours. Gene sequences are provided in Table S1.

Lin Y., Jin X., Nie Q., Chen M., Guo W., Chen L., Li Y., Chen X., Zhang W., Chen H., Jiang M., Xiao H., Zhang J., Fu F, & Wang C. (2022). YTHDF3 facilitates triple-negative breast cancer progression and metastasis by stabilizing ZEB1 mRNA in an m6A-dependent manner. Annals of Translational Medicine, 10(2), 83.

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