HL-60 (acute promyelocytic leukemia cell line), THP-1 (acute monocytic leukemia cell line), OCI-AML2 (acute myelomonocytic leukemia cell line) and OCI-AML3 (acute myelomonocytic leukemia cell line) cell lines were purchased from Shanghai Cell Bank of the Chinese Academy of Sciences. KASUMI-1 (acute myeloblastic leukemia cell line) cell line was gifted by Professor Chen Saijuan (Shanghai Institute of Hematology, Shanghai, China). These cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) (Gibco) at 37 °C in a humidified incubator containing 5% CO2. MV4-11(acute myelomonocytic leukemia cell line) and MOLM-13(acute monocytic leukemia cell line) cell lines were a kind gift from Professor Ravi Bhatia (City of Hope National Medical Center, Duarte, CA, USA). These two cell lines were cultured in Iscove's Modified Dulbecco's Medium (IMDM) supplemented with 10% (FBS). Diagnostic AML patient samples were purified by standard Ficoll‐Hypaque (Sigma-Aldrich) density centrifugation, then cultured in RPMI 1640 with 10% FBS.
Oci aml3
Manufactured by Shanghai Cell Bank
Sourced in United States
The OCI-AML3 is a cell line derived from a patient with acute myeloid leukemia. It is a commonly used in vitro model for research and development in the field of hematological malignancies.
Automatically generated - may contain errors
Lab products found in correlation
Ficoll hypaque, by Merck Group (4 mentions)
Thp 1, by Shanghai Cell Bank (4 mentions)
Oci aml2, by Shanghai Cell Bank (3 mentions)
Hl 60, by Shanghai Cell Bank (2 mentions)
Abt 199, by Merck Group (2 mentions)
St1326, by Merck Group (2 mentions)
Mg132, by Selleck Chemicals (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Iscove modified dulbecco media (imdm), by Thermo Fisher Scientific (1 mentions)
Thp 1, by Thermo Fisher Scientific (1 mentions)
Hl 60, by Thermo Fisher Scientific (1 mentions)
4 protocols using oci aml3
1
Culturing Diverse Acute Leukemia Cell Lines
2
Cultivation and Isolation of AML Cell Lines
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MV4-11 cell line was kindly endowed by Professor R. Bhatia (City of Hope National Medical Center, Duarte, CA, USA). THP-1, HL-60, U937 and OCI-AML3 cell lines were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences. Kasumi-1 cell line was gifted by Professor Chen Saijuan (Shanghai Institute of Hematology, Shanghai, China). MV4-11 cells were maintained in Iscove’s Modified Dulbecco’s medium (IMDM, Gibco, Billings, MT, USA) supplemented with 10% fetal bovine serum (FBS, Gibco), and THP-1, HL-60, U937 and OCI-AML3 cells were cultured in Roswell Park Memorial Institute 1640 (RPMI 1640, Gibco) medium supplemented with 10% FBS at 37 °C in a humidified incubator containing 5% CO2.
Bone marrow samples were obtained from AML patients following written informed consent. Mononuclear cells were isolated by Ficoll-Hypaque (Sigma-Aldrich, St. Louis, MO, USA) density gradient centrifugation. The study protocol was approved by the Ethics Committee of the First Affiliated Hospital of Zhejiang University, China.
Bone marrow samples were obtained from AML patients following written informed consent. Mononuclear cells were isolated by Ficoll-Hypaque (Sigma-Aldrich, St. Louis, MO, USA) density gradient centrifugation. The study protocol was approved by the Ethics Committee of the First Affiliated Hospital of Zhejiang University, China.
3
Establishment of AML Cell Lines
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MG132 were purchased from Selleck Chemicals(Houston, Texas, USA). ST1326 and ABT199 was purchased from Sigma-Aldrich (St Louis, MO, USA) Cell culture HL-60, THP-1, OCI-AML2 and OCI-AML3 cell lines were purchased from Shanghai Cell Bank of the Chinese Academy of Sciences. KASUMI-1 cell line was gifted by Professor Chen Saijuan (Shanghai Institute of Hematology, Shanghai, China). These cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum(FBS)(Gibco) at 37 °C in a humidi ed incubator containing 5% CO2. MV4-11 and MOLM-13 cell lines were a kind gift from Professor Ravi Bhatia (City of Hope National Medical Center, Duarte, CA, USA). These two cell lines were cultured in Iscove's Modi ed Dulbecco's Medium (IMDM) supplemented with 10% (FBS).
Diagnostic AML patient samples were puri ed by standard Ficoll-Hypaque (Sigma-Aldrich) density centrifugation, then cultured in RPMI 1640 with 10% FBS.
Diagnostic AML patient samples were puri ed by standard Ficoll-Hypaque (Sigma-Aldrich) density centrifugation, then cultured in RPMI 1640 with 10% FBS.
4
Establishment of AML Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MG132 were purchased from Selleck Chemicals(Houston, Texas, USA). ST1326 and ABT199 was purchased from Sigma-Aldrich (St Louis, MO, USA) Cell culture HL-60, THP-1, OCI-AML2 and OCI-AML3 cell lines were purchased from Shanghai Cell Bank of the Chinese Academy of Sciences. KASUMI-1 cell line was gifted by Professor Chen Saijuan (Shanghai Institute of Hematology, Shanghai, China). These cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum(FBS)(Gibco) at 37 °C in a humidi ed incubator containing 5% CO2. MV4-11 and MOLM-13 cell lines were a kind gift from Professor Ravi Bhatia (City of Hope National Medical Center, Duarte, CA, USA). These two cell lines were cultured in Iscove's Modi ed Dulbecco's Medium (IMDM) supplemented with 10% (FBS).
Diagnostic AML patient samples were puri ed by standard Ficoll-Hypaque (Sigma-Aldrich) density centrifugation, then cultured in RPMI 1640 with 10% FBS.
Diagnostic AML patient samples were puri ed by standard Ficoll-Hypaque (Sigma-Aldrich) density centrifugation, then cultured in RPMI 1640 with 10% FBS.
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