The largest database of trusted experimental protocols

Blocking antibody for mouse il 1β

Manufactured by Leinco Technologies

Blocking antibody for mouse IL-1β. This product is designed to neutralize the activity of mouse interleukin-1 beta (IL-1β) in in vitro experiments.

Automatically generated - may contain errors

2 protocols using blocking antibody for mouse il 1β

1

Bacterial Endotoxin and Cytokine Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ultrapure Escherichia coli O111:B4 LPS and CpG were from Invivogen. Recombinant mouse IL-23 and IL-1β, neutralizatiing antibody for IL-23p19 and isotype control (rat IgG) were purchased from eBioscience. Blocking antibody for mouse IL-1β was obtained from Leinco Technologies. Kanamycin (Km)-resistant wild-type Citrobacter rodentium strain DBS120 (pCRP1::Tn5) was a gift of Dr. David Schauer (Massachusetts Institute of Technology, Massachusetts). The isogenic C. rodentium Δler, Δtir, Δeae, ΔescN, ΔescU mutants were generated from DBS120. Salmonella enterica serovar Typhimurium strain SL1344 was a gift from Dr. D. Monack (Stanford University, California). For preparation of heat-killed C. rodentium, the pathogen was cultured overnight in LB medium, inoculated into DMEM at a 1:50 dilution and cultured under standard cell culture conditions (37°C with 5% CO2) for 8 hours without shaking. Bacteria were then harvested and washed twice with ice-cold PBS, and heat inactivated at 60°C for 30 min. Complete killing of bacteria was confirmed by 72 h incubation at 37°C on bacteria growth plates.
+ Open protocol
+ Expand
2

Pathogen Preparation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ultrapure Escherichia coli O111:B4 LPS and CpG were from Invivogen. Recombinant mouse IL-23 and IL-1β, neutralizating antibody for IL-23p19 and isotype control (rat IgG) were purchased from eBioscience. Blocking antibody for mouse IL-1β was obtained from Leinco Technologies. Kanamycin (Km)-resistant wild-type C. rodentium strain DBS120 (pCRP1::Tn5) was a gift of Dr David Schauer (Massachusetts Institute of Technology, Massachusetts). The isogenic C. rodentium Δler, Δtir, Δeae, ΔescN, ΔescU mutants were generated from DBS120. Salmonella enterica serovar Typhimurium strain SL1344 was a gift from Dr D. Monack (Stanford University, California). For preparation of heat-killed C. rodentium, the pathogen was cultured overnight in Luria-Bertani (LB) medium, inoculated into DMEM at a 1:50 dilution and cultured under standard cell culture conditions (37 °C with 5% CO2) for 8 h without shaking. Bacteria were then harvested and washed twice with ice-cold PBS, and heat inactivated at 60°C for 30 min. Complete killing of bacteria was confirmed by 72 h incubation at 37 °C on bacteria growth plates.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!