Enzyme immunoassay

Manufactured by Bio-Rad

Enzyme immunoassay is a type of analytical biochemistry technique that uses enzymes to detect and quantify specific molecules, typically proteins or hormones, in a sample. It relies on the high specificity of antibody-antigen interactions to identify and measure target analytes. The enzyme component, linked to the antibody, generates a detectable signal that is proportional to the concentration of the target molecule, allowing for quantitative analysis.

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Lab products found in correlation

Fungitell assay, by Associates of Cape Cod (1 mentions)

6 protocols using enzyme immunoassay

Galactomannan and β-D-Glucan Diagnostic Assay

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The galactomannan (GM) index was determined by enzyme immunoassay (BioRad) according to the manufacturer’s recommendations. A result was considered positive after two determinations (performed on two different assays but on the same sample) showing both an index equal to or greater than 0.5 for serum and an index equal to or greater than 1 for respiratory samples. β-d-glucan research was performed in serum using the Fungitell assay in duplicate (Associates of Cape Cod) following the manufacturer’s recommendations. A positive result was defined as two consecutive tests above a cutoff of 80 pg/ml.

Fekkar A., Lampros A., Mayaux J., Poignon C., Demeret S., Constantin J.M., Marcelin A.G., Monsel A., Luyt C.E, & Blaize M. (2021). Occurrence of Invasive Pulmonary Fungal Infections in Patients with Severe COVID-19 Admitted to the ICU. American Journal of Respiratory and Critical Care Medicine, 203(3), 307-317.

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Estimating Donor EBV Serology Profiles

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Donor VCA and EBNA-1 IgG serology were not available for all recipients. To estimate the EBV serology profiles of donors relevant to the present study, we assessed VCA and EBNA-1 IgG among deceased donors evaluated in the donor testing laboratory for the local organ procurement organization (LifeCenter Northwest). EBV serology, measured using the Bio-rad enzyme immunoassay (Bio-rad, Redmond, WA), was available from donors evaluated between February 16, 2011 through April 30, 2018

Heldman M.R., Edlefsen K.L., Pepper G., Kapnadak S.G., Rakita R.M., Fisher C.E, & Limaye A.P. (2022). Combined assessment of Epstein-Barr virus VCA and EBNA-1 serology for post-transplant lymphoproliferative disorder risk stratification in adult solid organ transplant recipients. Transplant infectious disease : an official journal of the Transplantation Society, 24(6), e13933.

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Detecting Viral Hepatitis Markers

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Testing for Hepatitis B virus surface antigen (HBsAg), antibody to Hepatitis B core antigen (anti-HBc), and antibody to Hepatitis C virus (anti-HCV) was performed by the SAIC NCI-Frederick National Laboratory. HBsAg was tested using an enzyme immunoassay from Bio-Rad Laboratories (Redmond, WA), and anti-HBC and anti-HCV were tested using enzyme-linked immunosorbent assays from Ortho-Clinical Diagnostics (Raritan, NJ). Concordance of samples with known HBV and HCV status were perfect.

Murphy G., Michel A., Taylor P.R., Albanes D., Weinstein S.J., Virtamo J., Parisi D., Snyder K., Butt J., McGlynn K.A., Koshiol J., Pawlita M., Lai G.Y., Abnet C.C., Dawsey S.M, & Freedman N.D. (2014). Association of seropositivity to Helicobacter species and biliary tract cancer in the ATBC study. Hepatology (Baltimore, Md.), 60(6), 1963-1971.

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Enzyme Immunoassay for GM Index

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The GM index was determined by enzyme immuno-assay (Bio-Rad) according to the manufacturer's recommendations. A result was considered positive after two determinations, performed on two different assays but on the same sample, showing both an index above 0.5 for serum and 1 for BAL.

Imbert S., Gauthier L., Joly I., Brossas J.Y., Uzunov M., Touafek F., Brun S., Mazier D., Datry A., Gay F, & Fekkar A. (2016). Aspergillus PCR in serum for the diagnosis, follow-up and prognosis of invasive aspergillosis in neutropenic and nonneutropenic patients. Clinical Microbiology and Infection, 22(6), 562.e1-562.e8.

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Enzyme Immunoassay for GM Index

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The GM index was determined by enzyme immunoassay (BioRad) according to the manufacturer’s recommendations. A result was considered positive after two determinations, performed on two different assays but on the same sample, showing both an index equal to or greater than 0.5 for serum and an index equal to or greater than 1 for BAL.

Imbert S., Meyer I., Palous M., Brossas J.Y., Uzunov M., Touafek F., Gay F., Trosini-Desert V, & Fekkar A. (2018). Aspergillus PCR in Bronchoalveolar Lavage Fluid for the Diagnosis and Prognosis of Aspergillosis in Patients With Hematological and Non-hematological Conditions. Frontiers in Microbiology, 9, 1877.

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Serological Markers of H. pylori and Viral Hepatitis

Cited 2 times

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Seropositivity for H. pylori, defined using 15 specific H. pylori antigens, was previously measured using a multiplex serology assay in a subset of the ATBC study participants. Seropositivity for ≥4 antigens was defined as being H. pylori seropositive. Details of the assay, including the assay validation and sensitivity, have been previously described [13 (link), 15 (link)].
Assays to determine hepatitis B virus surface antigen (HBsAg), antibody to hepatitis B core antigen (anti-HBc), and antibody to hepatitis C virus (anti-HCV) were previously conducted at the SAIC NCI-Frederick National Laboratory [16 (link)]. HBsAg was determined using an enzyme immunoassay from Bio-Rad Laboratories (Redmond, WA). Anti-HBc and anti-HCV were determined using ELISAs from Ortho-Clinical Diagnostics (Raritan, NJ).

Yang B., Petrick J.L., Abnet C.C., Graubard B.I., Murphy G., Weinstein S.J., Männistö S., Albanes D, & McGlynn K.A. (2017). Tooth loss and liver cancer incidence in a Finnish cohort. Cancer causes & control : CCC, 28(8), 899-904.

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