Proteinsimple ella assay

Manufactured by Bio-Techne

Sourced in United States

The ProteinSimple Ella assay is a microfluidic-based platform that enables automated, quantitative analysis of proteins in small sample volumes. It provides a simplified workflow for immunoassays, allowing for efficient and precise protein quantification.

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Lab products found in correlation

Magpix system, by Merck Group (2 mentions) Luminex xmap platform, by Merck Group (1 mentions) Multiplex magnetic bead based immunoassays, by R&D Systems (1 mentions) Luminex xmap multianalyte profiling platform, by Merck Group (1 mentions)

Close spelling variants of this product

ProteinSimple (34 citations) ProteinSimple Ella (10 citations)

2 protocols using proteinsimple ella assay

Quantifying Circulating Proteins in Blood

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Circulating proteins of interest were quantified from blood plasma using a ProteinSimple Ella assay (Bio-Techne) and a Luminex xMAP platform analyzed on the MAGPIX system (Merck Millipore) according to the manufacturer’s instructions. Protein levels measured with the Ella assay and xMAP platform are reported as pg/mL. Additionally, plasma samples were sent to Olink Proteomics AB for analysis and are reported as normalized protein expression levels calculated on a log₂ scale.

Englund D.A., Jolliffe A.M., Hanson G.J., Aversa Z., Zhang X., Jiang X., White T.A., Zhang L., Monroe D.G., Robbins P.D., Niedernhofer L.J., Kamenecka T.M., Khosla S, & LeBrasseur N.K. (2024). Senotherapeutic drug treatment ameliorates chemotherapy-induced cachexia. JCI Insight, 9(2), e169512.

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Senescence Protein Quantification in Mice

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To quantify the concentrations of senescence-related proteins, blood plasma from female (n = 2–6/group) and male (n = 2–6/group) mice was analyzed using commercially available multiplex magnetic bead-based immunoassays (R&D Systems) on the Luminex xMAP multianalyte profiling platform and analyzed on MAGPIX System (Merck Millipore). All assays were performed according to the manufacturer's protocols. The biomarkers quantified with this method include: GDF15, MMP2, MMP3, OPN, PAI1, and TNFR1. Proteins that did not reach reliable detection levels on the Luminex platform were run on ProteinSimple Ella assay (Bio-Techne, San Jose, CA, USA), in a subset of samples that had adequate volumes of remaining plasma. The biomarkers quantified with this method include TNF-alpha, IL6, and CCL2. All reported protein concentrations had over 80% detectability across samples.

Englund D.A., Jolliffe A., Aversa Z., Zhang X., Sturmlechner I., Sakamoto A.E., Zeidler J.D., Warner G.M., McNinch C., White T.A., Chini E.N., Baker D.J., van Deursen J.M, & LeBrasseur N.K. (2022). p21 induces a senescence program and skeletal muscle dysfunction. Molecular Metabolism, 67, 101652.

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