PDGCs lentivirally infected with a dox-inducible CD97-overexpression or empty vector were transfected with luciferase signaling-reporter plasmids: cAMP response element–Luciferase (Cat# E8471, Promega), SRE-Luciferase (Cat# E1340, Promega), SRF-RE-Luciferase (Cat# E1350, Promega), and NFAT-RE-Luciferase (Cat# E8481, Promega). Twenty-four hours after transfection, cells were reseeded in black 96-well plates at a density of 75,000 cells per well with medium containing doxycycline (or dox-free medium). Forty-eight hours after transfection, cells were lysed and luciferase activity was detected using the Bright-Glo Luciferase assay system (Cat# E2650, CisBio) and a BioTek Synergy H1 microplate reader according to the manufacturer’s protocol.
Bright glo luciferase assay system
Manufactured by PerkinElmer
The Bright-Glo Luciferase Assay System is a laboratory equipment designed to measure the activity of luciferase, an enzyme commonly used as a reporter in various biological assays. The system provides a simple, sensitive, and rapid method for quantifying luciferase activity in cell-based or cell-free systems.
Automatically generated - may contain errors
2 protocols using bright glo luciferase assay system
1
CD97-Mediated Transcriptional Regulation Assay
2
GPR133 Signaling Pathway Analysis
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HEK293T cells were cotransfected with GPR133 overexpression plasmids and either one of the following luciferase signaling-reporter plasmids: cAMP response element–Luciferase (Cat# E8471, Promega), SRE-Luciferase (Cat# E1340, Promega), SRF-RE-Luciferase (Cat# E1350, Promega), NFAT-RE-Luciferase (Cat# E8481, Promega), and NFκB-RE-Luciferase (Cat# E8491, Promega). Twenty-four hours after transfection, cells were reseeded in black 96-well plates at a density of 75,000 cells per well. Forty-eight hours after transfection, cells were lysed and luciferase activity was detected using the Bright-Glo Luciferase assay system (Cat# E2650, CisBio) and a BioTek Synergy H1 microplate reader according to the manufacturer’s protocol.
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