Sections of adjacent and THCA tissues were fixed in 4 % paraformaldehyde, embedded in paraffin, and sectioned to a thickness of 6 μm. For immunohistochemistry staining, sections were dewaxed, rehydrated, and treated with Tris-EDTA buffer solution (containing 10 mM Tris-Hcl and 1mM EDTA) to unmask the antigen and inactivate endogenous peroxidase. The sections were heated to boiling point in a pressure cooker for 5 min. After 3 washes, sections were incubated with BSA for 30 min and subsequently incubated with anti-CDKN2A antibody (1:100 dilution; proteintech, China) overnight at 4 °C. On the second day, sections were incubated at room temperature with secondary antibody for 1 h. Finally, sections were stained with hematoxylin, dehydrated, and mounted using neutral resins.
Anti cdkn2a antibody
Manufactured by Proteintech
Sourced in China
The Anti-CDKN2A antibody is a laboratory reagent used for the detection and analysis of the CDKN2A protein. CDKN2A, also known as p16, is a important cell cycle regulator. This antibody can be used in various applications, such as Western blotting, immunohistochemistry, and immunocytochemistry, to study the expression and localization of the CDKN2A protein in biological samples.
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2 protocols using anti cdkn2a antibody
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Immunohistochemical Staining of CDKN2A
2
Immunofluorescent Detection of CDKN2A
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The tissues were sectioned and embedded in paraffin. Sections were incubated overnight at 4°C with anti-CDKN2A antibody (1:200 dilution; proteintech, China). Slides were washed with phosphate-buffered saline (PBS) and incubated with a goat anti-rabbit IgG secondary antibody conjugated with fluorescein isothiocyanate (ZSDB-BIO, China) for 30 min with washed slides. After washing with PBS, they were incubated with an antifade reagent (Invitrogen, United States). Staining was visualized to determine protein expression levels using an Olympus CX41 fluorescence microscope (Olympus, Japan). The analysis results were performed using ImageJ software.
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