Ecg bioamplifier

Electrocardiograms (ECG) on anesthetized mice were obtained from needle electrodes inserted subcutaneously into each limb. Multiple leads were recorded for 3 minutes at 2 MHz. Electrocardiographic signals were amplified with an ADInstruments ECG Bioamplifier (Colorado Springs, CO), converted from analog to digital with an ACQ-16 Acquisition Interface and recorded with Ponemah Physiology Platform software (Gould; Valley View, OH). Recordings were analyzed using the ECG module of LabChart 5 software (AD Instruments).
For ambulatory ECG monitoring, 4- to 12-week-old mice were anesthetized with isofluorane, and telemetry transmitters (ETA-F10; DSI) were implanted in the back with leads tunneled to the right upper and left lower thorax. Heart rate, PR, and QRS intervals were calculated using Ponemah Physiology Platform (DSI) from 24-hour recordings.

Electrocardiograms (ECG) on anesthetized mice (as previously described above) were obtained from needle electrodes inserted subcutaneously into each limb. Multiple leads were recorded for 3 min at 2 MHz. Electrocardiographic signals were amplified with an ECG Bioamplifier (AD Instruments; Colorado Springs, CO), converted from analog to digital with an ACQ-16 Acquisition Interface and recorded with Ponemah Physiology Platform software (Gould; Valley View, OH). Recordings were analyzed using the ECG module of LabChart 5 software (AD Instruments).