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Vic dye labeled probe

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The VIC dye labeled probe is a fluorescent DNA probe used in real-time PCR experiments. It is designed to specifically bind to and detect target DNA sequences. The VIC dye emits a fluorescent signal when excited, allowing for the quantification of the target DNA during the PCR process.

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Lab products found in correlation

Quantstudio 6 flex, by Thermo Fisher Scientific (2 mentions) Rneasy kit, by Qiagen (2 mentions) High capacity rna to cdna kit, by Thermo Fisher Scientific (2 mentions) Fam dye labeled probes, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

VIC-labeled probe (13 citations) VIC probe (6 citations) VIC™ dye (3 citations)

2 protocols using vic dye labeled probe

1

Gene Expression Analysis by qPCR

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted using a RNeasy kit (QIAGEN) and reverse-transcription was done using the High Capacity RNA-to-cDNA kit (Thermo Fisher). Real-time quantitative PCR was done on a QuantStudio 6 Flex (Thermo Fisher) using TaqMan PCR (Applied Biosciences). FAM dye labeled probes (Thermo Fisher) were used against KRAS, CHEK1, MYC, and RIF1 and a VIC dye labeled probe was against ACTB, as a house-keeping control. Delta-delta CT was calculated for each sample. All samples were run as technical duplicates which were then standardized to their respective NS or vehicle treated controls. Means and standard deviations were calculated from the standardized values of three to four biological replicates.
Klomp J.E., Lee Y.S., Goodwin C.M., Papke B., Klomp J.A., Waters A.M., Stalnecker C.A., DeLiberty J.M., Drizyte-Miller K., Yang R., Diehl J.N., Yin H.H., Pierobon M., Baldelli E., Ryan M.B., Li S., Peterson J., Smith A.R., Neal J.T., McCormick A.K., Kuo C.J., Counter C.M., Petricoin EF I.I.I., Cox A.D., Bryant K.L, & Der C.J. (2021). CHK1 protects oncogenic KRAS-expressing cells from DNA damage and is a target for pancreatic cancer treatment. Cell reports, 37(9), 110060.
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2

Gene Expression Analysis by qPCR

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted using a RNeasy kit (QIAGEN) and reverse-transcription was done using the High Capacity RNA-to-cDNA kit (Thermo Fisher). Real-time quantitative PCR was done on a QuantStudio 6 Flex (Thermo Fisher) using TaqMan PCR (Applied Biosciences). FAM dye labeled probes (Thermo Fisher) were used against KRAS, CHEK1, MYC, and RIF1 and a VIC dye labeled probe was against ACTB, as a house-keeping control. Delta-delta CT was calculated for each sample. All samples were run as technical duplicates which were then standardized to their respective NS or vehicle treated controls. Means and standard deviations were calculated from the standardized values of three to four biological replicates.
Klomp J.E., Lee Y.S., Goodwin C.M., Papke B., Klomp J.A., Waters A.M., Stalnecker C.A., DeLiberty J.M., Drizyte-Miller K., Yang R., Diehl J.N., Yin H.H., Pierobon M., Baldelli E., Ryan M.B., Li S., Peterson J., Smith A.R., Neal J.T., McCormick A.K., Kuo C.J., Counter C.M., Petricoin EF I.I.I., Cox A.D., Bryant K.L, & Der C.J. (2021). CHK1 protects oncogenic KRAS-expressing cells from DNA damage and is a target for pancreatic cancer treatment. Cell reports, 37(9), 110060.
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