The trypsinized cells (1 × 106) were fixed with 75% ethanol at 4°C for 24 h. The fixed cells were incubated with RNase A (for 30 min at 374°C) after being washed with PBS, and 5 ml of PI (Beyotime, China) was added to the cell suspension. The mixture was incubated at room temperature for 30 min in the dark. The suspended cells were analyzed for cell cycle using flow cytometry (FACSCelesta 2, BD Biosciences). The percentages of cells in the G0/G1, S, and G2/M phases were counted and compared.
Facscelesta 2
Manufactured by BD
The FACSCelesta 2 is a flow cytometry instrument developed by BD. It is designed to analyze and sort cells based on their physical and fluorescent characteristics. The FACSCelesta 2 is capable of detecting and measuring multiple fluorescent parameters simultaneously.
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Lab products found in correlation
2 protocols using facscelesta 2
1
Cell Cycle Analysis by Flow Cytometry
2
Cell Cycle Analysis by Flow Cytometry
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The trypsinized cells (1×106) were xed with 75% ethanol at 4℃ for 24 h. The xed cells were incubated with RNase A (for 30 min at 374℃) after being washed with PBS, and 5 mL of PI (Beyotime, China) was added to the cell suspension. The mixture was incubated at room temperature for 30 min in the dark. The suspended cells were analyzed for cell cycle using ow cytometry (FACSCelesta 2, BD Biosciences). The percentages of cells in the G0/G1, S, and G2/M phases were counted and compared.
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