Carboplatin

Metformin (Santa Cruz Biotechnology, USA) and curcumin (Merck, Germany) were dissolved in RPMI 1640 culture medium (Bio-Idea, Iran) and DMSO (Shellmax, China), respectively. Anticancer drugs cisplatin (Mylan, USA), carboplatin (Mylan, USA), oxaliplatin (Sobhan, Iran), epirubicin (Ebewe, Austria), doxorubicin (Ebewe, Austria), docetaxel (Sanofi, France), paclitaxel (Sobhan, Iran), and methotrexate (Mylan, USA) were used. Cell proliferation kit (MTT) was provided from Roche (Switzerland). Matrigel Basement Membrane Matrix Growth Factor Reduced (Cat No. 354230) and Transwells (Cat No. 3422) were products of Corning (USA). Human AGS gastric cell line and human HDF normal cell line were purchased from the American Type Culture Collection (ATCC), were cultivated in RPMI 1640 and DMEM/F12 culture media, respectively, supplemented with heat-inactivated fetal bovine serum (FBS, Gibco, USA, 10 % v/v), 100 units/mL penicillin, and 100 μg/mL streptomycin (Bio-Idea, Iran).

Paclitaxel (Taxol^®, 6 mg/ml, Bristol-Myers Squibb, Egypt) and Carboplatin (Carboplatin^®10 mg/ml, MYLAN, France) were purchased from Egyptian market.
Gallic acid was purchased from Sigma Aldrich, USA. 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide dye was purchased from BIO BASIC, Canada. An Annexin V-fluorescein isothiocyanate (FITC)/Propidium iodide (PI) double staining kit was purchased from BioVision Research Products, USA. A RNeasy Mini Kit was purchased from QIAGEN, Venlo, Netherlands and a One-Step RT-PCR Kit was purchased from Bio-Rad, California, USA. Primary and secondary antibodies for western blotting were purchased from Cell Signaling Technology, Massachusetts, USA and Danko, California, USA, respectively. Materials and chemicals for electrophoresis were obtained from Bio-Rad (California, USA), Sigma Aldrich, USA and Roche, Basel, Switzerland.