The collected HF samples were embedded in paraffin sections. Then, the sections were sealed with bovine serum albumin and incubated overnight with the primary antibody at 4°C. The antibodies are ZYX (1:500, ab109316; Abcam, United Kingdom) and Ki67 (1:300, ab39012; Abcam). Then, the sections were incubated with a second antibody at room temperature for 1 h. After the second antibody was removed, the nuclei were counterstained with 4-amino-6-diamino-2-phenyl indole (DAPI, Beyotime Biotechnology, China). The stained sections were observed under a microscope (Olympus, Tokyo, Japan), and images were captured.
4 amino 6 diamino 2 phenyl indole dapi
Manufactured by Beyotime
Sourced in China
4-amino-6-diamino-2-phenyl indole (DAPI) is a fluorescent dye commonly used in various laboratory applications. DAPI selectively binds to DNA, specifically to the minor groove of double-stranded DNA, emitting fluorescence when excited by ultraviolet light. This property makes DAPI a valuable tool for staining and visualizing DNA in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Ab39012, by Abcam (1 mentions)
Ab109316, by Abcam (1 mentions)
A microscope, by Olympus (1 mentions)
Lsm 5 pascal laser scanning microscope, by Zeiss (1 mentions)
α sma, by Abcam (1 mentions)
Zyxin, by Abcam (1 mentions)
Ab184714, by Abcam (1 mentions)
Ab180711, by Abcam (1 mentions)
Sc 271835, by Santa Cruz Biotechnology (1 mentions)
3 protocols using 4 amino 6 diamino 2 phenyl indole dapi
1
Immunohistochemical analysis of HF samples
2
Dual Immunofluorescence Staining of α-SMA/Zyxin and s100a4/Zyxin
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Dual immunofluorescence staining experiments for α-SMA/Zyxin and s100a4 /Zyxin were performed on paraffin sections. The sections were sealed with bovine serum albumin and incubated overnight at 4°C with the respective primary antibody, including α-SMA (1:250, Abcam, UK), s100a4(1:100, Abcam, UK), and Zyxin (1:500, Abcam, UK). Then, the sections were incubated with a second antibody at room temperature for 1 h. After the second antibody was removed, the nuclei were counterstained with 4-amino-6-diamino-2-phenyl indole (DAPI, Beyotime Biotechnology, China). Fluorescence confocal images were captured using an LSM 5 Pascal Laser Scanning Microscope (Zeiss, Germany).
3
Immunohistochemical Analysis of Hypoxia Markers
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HF samples were embedded in paraffin. Next, histological sections were incubated overnight at 4 °C with primary antibodies for either EGLN1 (1 : 300, sc‐271835; Santa Cruz Biotechnology, Santa Cruz, CA, USA), EGLN3 (1 : 250, ab184714; Abcam, Cambridge, UK), PEDF (1 : 500, ab180711; Abcam) or SFPR2 (1 : 500, ab92667; Abcam). The sections were then incubated with the corresponding secondary antibody for 1 h at room temperature. After removing the secondary antibody, the nucleus was counterstained with 4‐amino‐6‐diamino‐2‐phenylindole (DAPI; Beyotime Biotech, Nanjing, China). The stained sections were observed under a microscope (Olympus, Tokyo, Japan), and representative images were captured.
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