Ab151432

Western immunoblot assays were performed by separating cell lysates on 4–12% sodium dodecyl sulfate (SDS) polyacrylamide gels (Novex) to separate proteins. The proteins were transferred to polyvinylidene difluoride (PVDF) membranes (Pierce; Thermo Fisher Scientific, Inc.), and the membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.05% Tween-20 (TBST). Membranes were subsequently incubated overnight at 4 °C with anti-DCTPP1 (ab224051, Abcam), anti-QPRT (ab171944, Abcam), anti-KAT5 (ab151432, Abcam), anti-EP300 (ab14984, Abcam), and anti-GAPDH antibodies (ab181602, Abcam), followed by incubation with horseradish peroxidase-conjugated secondary antibodies at room temperature for 2 h. Protein bands were revealed using chemiluminescence according to procedures given by the enhanced chemiluminescence (ECL) detection kit (Pierce, Thermo Scientific, Rockford, IL, USA).

Samples and cells were collected for Western blotting as previously described. Western blot analysis was performed using the following antibodies: LHPP (NBP1-83273, Novus, 0.2 ug/ml), METTL14 (ab220030, Abcam, 1:1000), HIF-1α (ab243861, Abcam, 1:1000), β-ACTIN (ab8226, Abcam, 1:2000), Acetyl Lysine (ab190479, Abcam, 1:1000), P300 (ab10485, Abcam, 1:5000), TIP60 (ab151432, Abcam, 1:1000), GCN5 (ab282176, Abcam, 1:1000), PCAF (ab176316, Abcam, 1:1000), GLUT1 (ab115730, Abcam, 1:5000), c-Myc (ab32072, Abcam, 1:1000), PKM2 (ab137852, Abcam, 1:1000), ALDOLASE (ab252953, Abcam, 1:1000), ENOLASE1 (H00002023-M01, Novus, 1:500), GLS1 (H00002744-M01, Novus, 1:500), GSK-3β (phospho S9) (ab75814, Abcam, 1:5000), GSK-3β (ab32391, Abcam, 1:5000), β-CATENIN (phospho S37) (ab75777, Abcam, 1:500).