Oxazepam

Synthetic light and heavy labeled peptides (Table S5) were obtained from New England Peptides (Boston, MA) and Thermo-Fisher Scientific (Rockford, IL), respectively. 17-β-estradiol, trifluoperazine, 4-hydroxyindole, propofol, naloxone, oxazepam, chloroform, Optima MS-grade acetonitrile/methanol and formic acid were purchased from Fischer Scientific (Fair Lawn, NJ). 17-β-Estradiol 3-glucuronide was purchased from Cayman chemicals (Ann Arbor, MI), whereas 17-β-Estradiol 17-glucuronide was purchased from Steraloid Inc. (Newport, RI). propofol β-D-glucuronide, naloxone 3β-D-glucuronide and oxazepam-glucuronide were purchased from Sigma Aldrich, Inc. (St. Louis, MO). Ammonium bicarbonate (98% purity) and sodium deoxycholate (98% purity) were obtained from Thermo Fisher Scientific (Rockford, IL) and MP Biomedicals (Santa Ana, CA), respectively.

Testosterone, alamethicin, UDP-glucuronic acid (UDPGA), magnesium chloride, dibasic and monobasic potassium phosphate were purchased from Sigma-Aldrich (St. Louis, MO). Oxazepam was purchased from Fischer Scientific (Fair Lawn, NJ), and tyrosine kinase inhibitors (TKIs) were kindly provided by Genentech (San Francisco, CA). Testosterone metabolites, respective conjugates, and standards were purchased from Cerilliant Corporation (Round Rock, TX) and Steraloid (Newport, RI). Bovine serum albumin (BSA) and protein extraction reagents were obtained from Thermo Fisher Scientific (Rockford, IL). Protein quantification chemicals and reagents were obtained as previously reported [33 (link)]. Hepatocyte thawing (MCHT50) and incubation (MM250) media were obtained from Lonza (Durham, NC), whereas, enterocytes thawing (CERM) and incubation (HQM) media were from IVAL (Columbia, MD). Synthetic light (with amino acid analysis) and heavy labeled peptides were purchased from New England Peptides (Boston, MA) and Thermo Fisher Scientific (Rockford, IL), respectively.