The HPLC-DAD-MS analyses of the phenolic extracts were performed according to a method described by Balli et al. [25 (link)]. The HPLC-DAD-MS system was from Waters, and comprised a 2695 HPLC, 2996 PAD, and 4 microtm MS equipped with Zspray ESI source. The ESI interface parameters were capillary 2.90 kV, cone 64 V in the first 18 min and 30 V until the end of the analysis, source temperature 120 °C, desolvation temperature 350 °C, cone gas flow 19 (L/Hr), and desolvation gas flow 350 (L/Hr). Data were acquired in negative ion mode from 110 m/z to 1000 m/z.
Hplc dad ms system
Manufactured by Waters Corporation
The HPLC-DAD-MS system is a high-performance liquid chromatography (HPLC) instrument that combines a diode-array detector (DAD) and a mass spectrometer (MS) for the analysis of complex samples. This system provides comprehensive analytical capabilities, including separation, identification, and quantification of a wide range of compounds.
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2 protocols using hplc dad ms system
1
HPLC-DAD-MS Analysis of Phenolic Extracts
2
Analysis of Phenolic and Steryl Ferulates
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All the extracts were analyzed using a HP 1200L liquid chromatograph equipped with a DAD detector (Agilent Technologies, Palo Alto, CA, USA) with a Raptor ARC-18 column (150 × 3 mm, 5 µm, Restek, USA). The gradient method for the phenolic extracts was the same proposed by The HPLC-DAD-MS analyses of phenolic and steryl ferulates extracts were performed using the same column and chromatographic conditions previously described. HPLC-DAD-MS system was from Waters and composed by 2695 HPLC, 2996 PAD and 4 micro MS equipped with Zspray ESI source. The ESI interface parameters were capillary 3.50 kV, cone 76 V, source temperature 120 °C, desolvation gas temperature 350 °C, cone gas flow 25 (L/h), desolvation gas flow 370 (L/h). Data were acquired in negative ion mode from 150 m/z to 800 m/z, at 0.5 sec/scan rate. The chromatograms were recorded at 330 nm.
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