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2 protocols using nbp235211

1

Immunoassay Reagents for Insulin Analysis

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Goat anti-mouse IgG (GTX77316, GeneTex), goat anti-rabbit IgG (GTX77061, GeneTex), streptavidin (85,878, Sigma Aldrich), streptavidin conjugation kit (ab102921, Abcam), Amicon® Ultra filters low-binding PES filter with 30 k molecular separation pores (UFC503096, Millipore), agarose powder (4,718, Sigma Aldrich), TAE buffer (Tris/Acetic Acid/EDTA) (1,610,743, Bio-Rad), SYBG Gold dye (41,003, Thermo Fisher Scientific), low–molecular weight DNA ladder (B7025, New England Biolabs Inc.), PowerPac™ basic power supply (1,645,050, Bio-Rad), Mini-Sub Cell GT Cell (1,704,406, Bio-Rad), Gel Doc™ EZ System (1708270EDU, Bio-Rad), SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices), recombinant human insulin (I2643, Sigma Aldrich), recombinant human IFN-γ (285-IF, R&D Systems), recombinant human IP10 (ab9810, Abcam), recombinant IL-6 (206-IL, R&D Systems), recombinant TNF-α (210-TA, R&D Systems), recombinant IGF-1 (291-G1, R&D Systems), recombinant human proinsulin C-peptide (NBP235211, Novus Biologicals), rabbit polyclonal anti-human insulin (ab53591, Abcam), rabbit polyclonal anti-human human insulin antibody (NBP1-87485, Novus Biologicals), rabbit polyclonal anti-human insulin (biotin) (ab53592, Abcam), mouse monoclonal anti-human insulin (ab6995, Abcam), and mouse monoclonal anti-human human insulin antibody (NBP100-73008, Novus Biologicals).
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2

Standardization of Recombinant Insulin Proteins

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The following recombinant human proteins were used for the standardization of experiments and as positive controls for immunoblots: Human Proinsulin C-peptide Analogue Protein (NBP2-35211, Novus). Recombinant human Proinsulin (1336-PN, R&D systems). Insulin Recombinant protein (NBP-1-99193, Novus).
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