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Z magna rip rna binding protein immunoprecipitation kit

Manufactured by Merck Group
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Sourced in United States

The Z-Magna RIP™ RNA-binding Protein Immunoprecipitation kit is a laboratory tool designed for the isolation and purification of RNA-binding proteins and their associated RNA transcripts. The kit provides the necessary components and protocols to perform this specialized procedure.

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Lab products found in correlation

Anti ago2, by Abcam (3 mentions) Anti eif4a3, by Abcam (1 mentions) Anti hnrnpc, by Abcam (1 mentions)

Close spelling variants of this product

Magna RIP Kit (692 citations) RIP kit (218 citations) Magna RNA-binding protein immunoprecipitation kit (149 citations) RNA-Binding Protein Immunoprecipitation Kit (125 citations) RNA Immunoprecipitation Kit (15 citations) Immunoprecipitation Kit (14 citations) RNA immunoprecipitation (RIP) kit (7 citations) Magna RNA RIP Kit (4 citations) Z-Magna RIPTM RNA-binding Protein Immunoprecipitation kit (3 citations)

3 protocols using z magna rip rna binding protein immunoprecipitation kit

1

Quantifying RNA-Protein Interactions

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Under the instruction of the manual of Z‐Magna RIP™ RNA‐binding protein immunoprecipitation kit (Millipore Corporation), RIP assay was done. Anti‐Ago2 (Abcam), anti‐EIF4A3 (Abcam) as well as anti‐IgG (Abcam) were added into cell lysates for incubation. Finally, the RNA‐protein complexes were purified and subjected to RT‐qPCR analysis. This experiment was performed in triplicate.
Zhou Q., Fu Q., Shaya M., Kugeluke Y., Li S, & Dilimulati Y. (2022). Knockdown of circ_0055412 promotes cisplatin sensitivity of glioma cells through modulation of CAPG and Wnt/β‐catenin signaling pathway. CNS Neuroscience & Therapeutics, 28(6), 884-896.
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2

RIP Assay for TM4SF19-AS1, miR-153-3p, LAMC1

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RIP assay was performed by employing Z-Magna RIP™ RNA-binding Protein Immunoprecipitation kit (Millipore, Bedford, MA, USA). RIP lysis buffer was added to lyse HNSCC cells. Afterward, cell lysates were co-cultivated with anti-IgG (Abcam), anti-Ago2 (Abcam) or anti-HNRNPC (Abcam). Eventually, the enrichment of TM4SF19-AS1, miR-153-3p and LAMC1 was examined by RT-qPCR analysis. The independent assay was performed three times.
Yu Z., Wang X., Niu K., Sun L, & Li D. (2022). LncRNA TM4SF19-AS1 exacerbates cell proliferation, migration, invasion, and EMT in head and neck squamous cell carcinoma via enhancing LAMC1 expression. Cancer Biology & Therapy, 23(1), 1-9.
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3

RIP Assay for Ago2 Interactions

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Z-Magna RIP™ RNA-binding Protein Immunoprecipitation kit (Millipore Corporation, USA) was adopted to perform RIP assay. Anti-Ago2 (Abcam) antibody as well as anti-IgG (Abcam) antibody were used for immunoprecipitation with cell lysates. Finally, the RNA complexes were subjected to extraction for RT-qPCR analysis. The experimental procedure was independently carried out in triplicate.
Zhang W., Liang F., Li Q., Sun H., Li F., Jiao Z, & Lei J. (2022). LncRNA MIR205HG accelerates cell proliferation, migration and invasion in hepatoblastoma through the activation of MAPK signaling pathway and PI3K/AKT signaling pathway. Biology Direct, 17, 2.
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