Single-cell suspensions from MC38-Vector or MC38-IL33 tumors were incubated with APC-conjugated CD11b, FITC-conjugated anti-Gr1, PE-conjugated anti-F4/80, or eFluor450-conjugated anti-CD45 (all from eBioscience, San Diego, CA). One million cells were incubated with antibodies in 100 μL wash buffer (PBS with 0.1% BSA) at 4°C for 1 h. Cells were washed twice with ice-cold wash buffer and fixed with 1% paraformaldehyde. FACS analyses were performed on a Beckton Dickinson LSRII flow cytometer and analyzed with FACSDiva 8.0 software (BD Biosciences).
Efluor450 conjugated anti cd45
Manufactured by Thermo Fisher Scientific
Sourced in United States
EFluor450-conjugated anti-CD45 is a laboratory reagent used to detect and analyze CD45-expressing cells. It consists of an anti-CD45 antibody conjugated to the EFluor450 fluorescent dye. This product can be used in flow cytometry and other immunoassays.
Automatically generated - may contain errors
Lab products found in correlation
Facsdiva 8, by BD (1 mentions)
Fitc conjugated anti gr 1, by Thermo Fisher Scientific (1 mentions)
Apc conjugated cd11b, by Thermo Fisher Scientific (1 mentions)
Pe conjugated anti f4 80, by Thermo Fisher Scientific (1 mentions)
Lsrii flow cytometer, by BD (1 mentions)
Apc conjugated anti f4 80 ab, by BD (1 mentions)
Fitc conjugated anti cd11b, by BD (1 mentions)
2 protocols using efluor450 conjugated anti cd45
1
Phenotyping tumor-infiltrating immune cells
2
Isolation and Analysis of Mouse Liver Macrophages
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The NPCs of mouse liver were isolated from chimera mice, then analyzed by flow cytometry as described previously [42 (link)]. To make gating strategies for analysis of macrophages, liver NPCs were stained with APC-conjugated anti-F4/80 Ab (BD Bioscience, Franklin Lakes, NJ, USA), eFluor 450-conjugated anti-CD45 (eBioscience, CA, USA), FITC-conjugated anti-CD11b (BD Bioscience, USA) or PE-conjugated anti-CD86 Ab (BD Bioscience, USA) for 15 min at 4 °C in the dark. The analysis was performed using FACS Calibur H (BD Bioscience).
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