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The UAS-ArmS10 is a genetic construct used in Drosophila research. It contains the UAS (Upstream Activation Sequence) regulatory element that can be used to induce targeted gene expression in a tissue-specific manner.

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Lab products found in correlation

Uas tkvqd, by Bloomington Drosophila Stock Center (1 mentions) W1118, by Bloomington Drosophila Stock Center (1 mentions) Tub gal80ts, by Bloomington Drosophila Stock Center (1 mentions) Uas hdac1rnai, by Bloomington Drosophila Stock Center (1 mentions) Uas mcd8 gfp, by Bloomington Drosophila Stock Center (1 mentions)

3 protocols using uas arms10

1

Genetic Toolkit for Wnt Signaling

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The following strains were obtained from repositories: TubGAL80ts; TM2/TM6b,Tb, UAS-FUCCI, UAS-Wnt2RNAi (29441), UAS-Wnt4RNAi (29442), UAS-Wnt5RNAi (28534), UAS-Wnt6RNAi (30493), UAS-WntDRNAi (28947), UAS-Wnt10RNAi (31989), UAS-CyclinBRNAi (34544), UAS-StgRNAi (29556), nkd-lacZ, Chk1-LacZ, fz3-GFP, UAS-ArmS10, UAS-TkvQD, Wnt6 KO, Wnt5400, Wgts (Bloomington Drosophila Stock Center), UAS-Chk1RNAi (110076), UAS-TCFRNAi (3014), UAS-Fz2RNAi (44391), UAS-WgRNAi (13352), UAS-Wnt10RNAi (100867), UAS-DshRNAi (101525), UAS-ArmRNAi (7767), UAS-Cdc2RNAi (106130) (Vienna Drosophila Resource Center), UAS-Chk1 (In-house fly facility). The following strains were received as gifts: btl-GAL4, UAS-ATR, UAS-Wnt10. Strains were raised on a diet of cornmeal-agar and maintained at 25°C except GAL80ts and Wgts strains that were maintained at 18°C. For experiments involving GAL80ts and Wgts strains, the animals were moved to 29°C at indicated stages for indicated time periods. All experiments were performed on animals raised at 25°C unless otherwise indicated.
Kizhedathu A., Kunnappallil R.S., Bagul A.V., Verma P, & Guha A. (2020). Multiple Wnts act synergistically to induce Chk1/Grapes expression and mediate G2 arrest in Drosophila tracheoblasts. eLife, 9, e57056.
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2

Genetic Manipulation of Wnt Signaling

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All stocks were maintained and crossed at 25°C according to standard procedures. UAS-Wnt4 was generated by using full-length cDNA of Wnt4 inserted into pUAST construct, followed by standard P-element transformation. Wnt4EMS23, Df(2L)BSC226, UAS-TCF-DN (also called UAS-TCFΔN), UAS-Wingless-RNAi, UAS-Arm-S10 are from the Bloomington stock center. UAS-Wnt4-RNAi is from VDRC (Transformant ID: 104671). UAS-Fz-DN is gift from Dr. Richard Carthew (Northwestern University) (Zhang and Carthew, 1998 (link)). UAS-DshDIX, UAS-Dsh ΔDEP are gifts from Dr. Jeffrey Axelrod (Stanford University) (Axelrod et al., 1998 (link)). Hand-Gal4 and Hand-GFP were generated and described in our previous studies (Han and Olson, 2005 (link); Han et al., 2006 (link)).
Chen Z., Zhu J.Y., Fu Y., Richman A, & Han Z. (2016). Wnt4 is required for ostia development in the Drosophila heart. Developmental biology, 413(2), 188-198.
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3

Drosophila Transgenic Line Protocols

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Fly lines were cultured at room temperature or 25–29 °C on standard fly food or food-plus-compound. Fly food contained tayo agar 10g, soya flour 5g, sucrose 15g, glucose 33g, maize meal 15g, wheat germ 10g, treacle molasses 30g, yeast 35g, nipagin 10ml, propionic acid 5ml in 1000 ml water. Transgenes used (Bloomington Drosophila Stock Center number): byn-gal4 (hindgut-specific line, V. Hartenstein), UAS-RasG12V (second chromosome, G. Halder), tub-gal80TS (Bloomington Drosophila Stock Center #7017), w1118 (#3605), UAS-mCD8-GFP (#5137), UAS-Hex-C-RNAi (#57404), UAS-UGP-RNAi (#50902), UAS-GlcAT-P-RNAi (#67771), UAS-sgl-RNAi (#65348), UAS-Akt-RNAi (#82957), UAS-plx-RNAi (AS160, #66313), UAS-HDAC1-RNAi (#36800), and UAS-ArmS10 (#4782).
Cong B., Thakur T., Uribe A.H., Stamou E., Gopinath S., Maddocks O, & Cagan R. (2023). Colon Cancer Cells Evade Drug Action by Enhancing Drug Metabolism. bioRxiv.
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