Vwf antigen

Manufactured by Siemens

Sourced in Germany

The VWF antigen is a laboratory equipment product designed to measure the levels of von Willebrand factor (VWF) in a sample. VWF is a blood plasma glycoprotein that plays a crucial role in the blood clotting process. The VWF antigen test provides a quantitative measurement of the VWF protein in the sample, which can be used to assist in the diagnosis and monitoring of various bleeding and clotting disorders.

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Lab products found in correlation

Bcs analyzer, by Siemens (1 mentions) Calibrated automated thrombogram, by Diagnostica Stago (1 mentions) Cs 5100i, by Sysmex (1 mentions)

2 protocols using vwf antigen

Comprehensive Blood Coagulation Analysis

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Blood samples were collected into 3 mL tubes (Sarstedt) containing 3.2% citrate. The samples were processed within 1 hour after the blood collection, centrifuged at 2000 g for 20 minutes to prepare platelet-poor plasma, aliquoted, and immediately stored at −80 °C. The aliquots were thawed directly before the analysis. Anti-Xa activity was determined with a chromogenic substrate and a bovine FXa (Hyphen BioMed), and a chromogenic anti-IIa assay with human thrombin (Hyphen BioMed) was used to measure anti-IIa activity. The FVIII activity was assessed by a one-stage clotting test (Siemens Healthineers). In addition, activities of D-dimer, prothrombin fragments, VWF antigen, and VWF (all purchased from Siemens Healthineers) were measured on a BCS analyzer (Siemens Healthineers). The TG assay was carried out on a Fluoroscan Ascent (Fisher Scientific) at a 390/460-nm wavelength using a calibrated automated thrombogram (Diagnostica Stago) activated with 5-pM tissue factor.

Pfrepper C., Koch E., Weise M., Siegemund R., Siegemund A., Petros S, & Metze M. (2023). Weight-adjusted dosing of tinzaparin for thromboprophylaxis in obese medical patients. Research and Practice in Thrombosis and Haemostasis, 7(2), 100054.

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Comparison of VWF Assays Across Sites

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At site 1, VWF assays were performed by Siemens VWF antigen and Innovance VWF Ac (Marburg, Germany), a GP1bM VWF activity assay.2 All assays were performed on Sysmex CS5100i instrumentation (Kobe, Japan). VWF:CB was performed by ELISA using Technozym CBA (Technoclone, Vienna, Austria).
At site 2, VWF assays were performed by VWF Ac or VWF:RCo, and VWF:Ag using ACL TOP750CT instrumentation (all Werfen, Barcelona, Spain). VWF:CB was performed by Euroanalyser one (Euro Diagnostic Systems, Tamil Nadu, India) using Zymutest VWF:CBA kit (Hyphen Biomed, Neuville‐sur‐Oise, France).

Bowyer A.E., Goodfellow K.J., Seidel H., Westhofen P., Stufano F., Goodeve A., Kitchen S, & Makris M. (2018). Evaluation of a semi‐automated von Willebrand factor multimer assay, the Hydragel 5 von Willebrand multimer, by two European Centers. Research and Practice in Thrombosis and Haemostasis, 2(4), 790-799.

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