Cells with and without serum starvation for 24 h were fixed and permeabilized. Cells were then stained with APO-BrdU TUNEL Assay Kit with Alexa Fluor 488 anti-Brdu (A23210, ThermoFisher Scientific) and LIVE/DEAD Fixable for Far Red Dead Cell Stain Kit, for 633 nm excitation (L34973, ThermoFisher Scientific). Apoptosis was assessed by FACS analysis using FACSCalibur (Becton Dickinson) under 488 nm and 635 nm lasers. Cells negative for both far-red dye and TUNEL staining are live cells; far-red-dye negative, TUNEL-positive staining cells are early apoptotic cells; far-red-dye positive, TUNEL-negative staining cells are dead cells; far-red-dye positive, TUNEL-positive staining cells are primarily cells in late stages of apoptosis. The total percentage of early and late apoptotic cells were compared.
Live dead fixable for far red dead cell stain kit
Manufactured by Thermo Fisher Scientific
The LIVE/DEAD Fixable for Far Red Dead Cell Stain Kit is a fluorescent dye-based solution used to detect dead cells in a cell population. The kit provides a simple, rapid, and effective method for identifying dead cells in a sample. The dye is cell-impermeant and only stains cells with compromised membranes, making it suitable for use in flow cytometry and other applications that require the detection of dead cells.
Automatically generated - may contain errors
2 protocols using live dead fixable for far red dead cell stain kit
1
Apoptosis Analysis by FACS
2
Apoptosis Quantification by Flow Cytometry
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