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Skge004b

Manufactured by R&D Systems
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Sourced in United States

The SKGE004B is a lab equipment product designed for research and experimentation purposes. It serves a core function of facilitating specific laboratory tasks, but a detailed description while maintaining an unbiased and factual approach is not available.

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Lab products found in correlation

Ldh cytotoxicity detection kit, by Takara Bio (1 mentions) Human tnf alpha quantikine elisa kit, by R&D Systems (1 mentions) Human il 10 quantikine elisa kit, by R&D Systems (1 mentions) Dy510, by R&D Systems (1 mentions) Dy506, by R&D Systems (1 mentions)

3 protocols using skge004b

1

Interplay of NPCs and P. acnes

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Nucleus pulposus cells (NPCs) were subcultured in 6-well plates, cocultured with P. acnes for different times and incubated with indicated components, respectively. The supernatant (1 ml) of the culture medium was collected to determine PGE2 concentrations by an enzyme-linked immunosorbent assay (ELISA) (SKGE004B, R&D Systems Inc., Minneapolis, MN, USA).
Lin Y., Tang G., Jiao Y., Yuan Y., Zheng Y., Chen Y., Xiao J., Li C., Chen Z, & Cao P. (2018). Propionibacterium acnes Induces Intervertebral Disc Degeneration by Promoting iNOS/NO and COX-2/PGE2 Activation via the ROS-Dependent NF-κB Pathway. Oxidative Medicine and Cellular Longevity, 2018, 3692752.
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2

Measurement of Cell Death Markers

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Apoptotic single stranded DNA (ssDNA) was quantified using an ssDNA monoclonal antibody (Enzo Life Sciences Cat# ALX-804-192, RRID:AB_10541559) as described previously (69 (link)). Lactate dehydrogenase (LDH) activity of supernatants was measured using the LDH cytotoxicity detection kit (Takara Bio) and percent cytotoxicity calculated according to the manufacturer’s protocol. PGE2 was quantified in cell culture supernatants using a forward sequential competitive enzyme immunoassay kit (R&D Systems, Cat# SKGE004B). TNF and IL-10 were quantified using the Human TNF-alpha Quantikine ELISA Kit (R&D Systems, Cat# STA00D) and the Human IL-10 Quantikine ELISA Kit (R&D Systems, Cat# S1000B), respectively.
Kuriyan J., Kong X.P., Krishna T.S., Sweet R.M., Murgolo N.J., Field H., Cerami A, & Henderson G.B. (1991). X-ray structure of trypanothione reductase from Crithidia fasciculata at 2.4-A resolution.. Proceedings of the National Academy of Sciences of the United States of America, 88(19), 8764-8768.
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3

Quantification of Inflammatory Markers in Brain Samples

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Levels of IL-6, PGE2 and TNF-α in brain samples (supernatants) were determined with specific ELISA kits according to the manufacturer’s protocols (R&D Systems, Minneapolis, MN, USA; catalog numbers DY506, SKGE004B and DY510, respectively). The detection limits of the assays were as follows: IL-6—125–8000 pg/mL, PGE2—39–2500 pg/mL, TNF-α—62.5–4000 pg/mL. Whenever the level of the tested constituent was less than the lower detection limit of the assay, results were marked as “undetectable” and given a value of zero.
Nassar A, & Azab A.N. (2022). Effects of Dexamethasone and Pentoxifylline on Mania-like and Depression-like Behaviors in Rats. Pharmaceuticals, 15(9), 1063.
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