Anti-C20 (1:1000) antibody used to detect APP and its β-CTFs was kindly provided by laboratory of Professor Weihong Song. Anti-BACE1 (1:1000, ab183612) and anti-PS1 (1:1000, ab76083) antibodies were purchased from Abcam (Cambridge, MA, USA). Anti-Ubquitin (1:1000, #10201-2-AP), anti-Beclin-1 (1:1000, #11306-1-AP), anti-Nrf2 (1:1000, #10396-1-AP), anti-HO-1(1:1000, #10701-1-AP), anti-SOD-1 (1:1000, #10269-1-AP), and anti-caspase-3 (1:1000, #66470-2-Ig) antibodies were obtained from Proteintech (Wuhan, Hubei, China). Anti-P62 (1:1000, H00008878-M01), anti-LC3 (1:1000, #12741) and anti-β-actin (1:3000, A5411) antibodies were purchased from Abnova (Taipei, Taiwan, China), Cell Signaling Technology (Danvers, MA, USA) and Sigma (St. Louis, MO, USA), respectively.
Anti lc3
Manufactured by Abnova
Sourced in China
Anti-LC3 is a laboratory product that detects the presence of the LC3 (microtubule-associated protein 1A/1B-light chain 3) protein. LC3 is a widely used marker for autophagy, a cellular process that involves the degradation and recycling of damaged or unwanted cellular components.
Automatically generated - may contain errors
Lab products found in correlation
Ab76083, by Abcam (1 mentions)
Ab183612, by Abcam (1 mentions)
Anti caspase 3, by Proteintech (1 mentions)
Anti β actin, by Abnova (1 mentions)
Anti p62, by Abnova (1 mentions)
Anti ho 1, by Proteintech (1 mentions)
Anti sod1, by Proteintech (1 mentions)
Anti beclin 1, by Proteintech (1 mentions)
Anti nrf2, by Proteintech (1 mentions)
Anti lamin a c, by Cell Signaling Technology (1 mentions)
Anti ctgf, by Abcam (1 mentions)
Anti rps6, by Cell Signaling Technology (1 mentions)
Anti lats1, by Cell Signaling Technology (1 mentions)
Anti p yap, by Cell Signaling Technology (1 mentions)
Cell lysis buffer, by Cell Signaling Technology (1 mentions)
Anti flag antibody, by Merck Group (1 mentions)
Anti tsc2, by Cell Signaling Technology (1 mentions)
Anti yap taz, by Merck Group (1 mentions)
Anti yap, by Merck Group (1 mentions)
Anti p62 sqstm, by Abnova (1 mentions)
2 protocols using anti lc3
1
Immunoblotting for APP, BACE1, and Oxidative Stress
2
Protein Extraction and Immunoblotting Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells and tissues were lysed in cell lysis buffer (#9803; Cell Signaling Technology) with complete protease inhibitor cocktail and phosSTOP phosphatase inhibitor cocktail (Roche). Protein extracts were resolved by SDS-PAGE, transferred to PVDF membranes and incubated with the following primary antibodies: anti-αSMA (#A2547; Sigma-Aldrich); anti-Mart1 (#sc-20032; Santa Cruz Biotechnology, Inc.); anti-GAPDH (#ab9485), anti-CTGF (#ab6992; Abcam); anti-AREG (#sc-74501Invitrogen); anti-TSC1 (#37-0400; Invitrogen); anti-LC3 (#0231-100), anti-p62/SQSTM (#H00008878-M01; Abnova); anti–β-actin (#A5441), anti–α-tubulin (#T5168; Sigma-Aldrich); anti-YAP (#4912), anti-YAP/TAZ (#8418), anti-Lamin A/C (#2032), anti-rpS6 (#2217), anti-phospho-rpS6(Ser240/244; #5364), anti-p4EBP1(Thr37/46; #2855), anti-pYAP (Ser127; #4911), anti-Lats1 (#3477), anti-TSC2 (#4308), and anti-Ub (#3936; Cell Signaling Technology). Nuclear and cytoplasmic protein extractions were performed with NE-PER kit (#78835; Thermo Fisher Scientific). Flag-tagged YAP protein complex was immunoprecipitated from cell lysates with anti-Flag antibody (#F1804; Sigma-Aldrich) and protein G–Sepharose (#17-0618-01; GE Healthcare). Immunocomplexes were washed in cell lysis buffer three times and analyzed by immunoblotting.
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