Mda mb 435 melanoma cells

A-10 embryonic rat aortic smooth muscle, HeLa cervical carcinoma, SK-OV-3 ovarian carcinoma, and MDA-MB-231 breast carcinoma cell lines were obtained directly from ATCC (Manassas, VA). The MDA-MB-435 melanoma cells were acquired from the Lombardi Cancer Center (Georgetown University) and validated by ATCC. NCI/ADR-RES cells were obtained from the NIH (Bethesda, MD). The SK-OV-3/MDR-1–6/6 and HeLa wild-type βIII (WT βIII) cell lines were described previously.^{17 (link)} MDA-MB-435, MDA-MB-231 and NCI/ADR-RES cells were cultured in Improved Minimum Essential Medium (Richter’s Modification) (Gibco, Life Technologies, Grand Island, NY) with 10% fetal bovine serum and 25 μg/mL gentamicin. WT βIII cells were grown in DMEM (Gibco, Life Technologies) with 10% fetal bovine serum and 50 μg/mL gentamicin, while all the other cell lines were maintained in Basal Medium Eagle (Sigma-Aldrich) with 10% fetal bovine serum and 50 μg/mL gentamicin. All cells were maintained in a humidified incubator at 37 °C with 5% CO₂.