The extracted total RNA was treated with Rnase R (Epicentre, Madison, WI, USA) to remove linear RNA. The enriched circRNAs were amplified and then transcribed into the fluorescent circRNAs using random primers according to the instructions for the CapitalBio cRNA Amplification and Labeling Kit (CapitalBio, Beijing, China). The amplified circRNA was purified utilizing an RNeasy Mini Kit (Qiagen, Dusseldorf, Germany). The concentration and specific activity of the labeled circRNAs were assessed using the NanoDrop ND-1000. Then the labeled circRNAs were hybridized onto the Agilent human circRNA array (version 1.0; Agilent Technologies, Santa Clara, CA, USA). The slides were rotated at a speed of 20 rpm and a temperature at 42°C in an Agilent hybridization oven overnight, washed, and scanned by the Agilent G2505C Scanner.
Agilent human circrna array
Manufactured by Agilent Technologies
Sourced in United States
The Agilent human circRNA array is a microarray platform designed to analyze the expression profiles of circular RNAs (circRNAs) in human samples. The array provides comprehensive coverage of known and predicted human circRNAs, allowing for the simultaneous detection and quantification of multiple circRNA species within a single experiment.
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2 protocols using agilent human circrna array
1
Profiling Circular RNAs via Microarray
2
Enrichment and Labeling of circRNAs
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Total extracted RNA was treated with Rnase R (Epicentre, Madison, WI, USA) to remove linear RNA. Enriched circRNAs were amplified and then transcribed into fluorescent circRNAs using random primers according to the instructions for the CapitalBio cRNA Amplification and Labeling Kit (CapitalBio, Beijing, China). Amplified circRNA was purified with the RNeasy Mini Kit (Qiagen, Dusseldorf, Germany). Concentration and specific activity of the labeled circRNAs were assessed using the Nano Drop ND-1000. The labeled circRNAs were then hybridized onto the Agilent human circRNA array (version 1.0; Agilent Technologies, Santa Clara, CA, USA). The slides were rotated at 20 rpm at 42°C in an Agilent hybridization oven overnight and were then washed and scanned using the Agilent G2505C Scanner.
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