Application software v4

Manufactured by Leica

Sourced in Germany

Leica Application Software V4.9 is a software package designed to work with Leica's laboratory equipment. It provides a user interface and tools for controlling and managing various Leica instruments and digital imaging systems. The software enables data acquisition, image processing, and instrument configuration.

Automatically generated - may contain errors

Lab products found in correlation

Coreldraw 11, by Corel (2 mentions) Bh 2 microscope, by Olympus (2 mentions) Dfc7000 t camera, by Leica (2 mentions) Methyl salicylate, by Merck Group (1 mentions)

Close spelling variants of this product

Leica Application Suite software (LAS) V4.12 Leica Application Suite software v4.5.0 V4.12.0 Leica Application Suite software Leica application software Leica Application

2 protocols using application software v4

Detailed Microscopic Examination of Tibial Innervation

Check if the same lab product or an alternative is used in the 5 most similar protocols

For microscopy, the cuticle was removed around the fixed tissue by carefully cutting at the dorsal and ventral side of the tibia along the tibia's main axis with a piece of a blade, and by removing the cuticle with forceps from the internal tissues and neuronal components. The leg preparations were mounted in methyl salicylate on a glass slide, viewed with an Olympus BH-2 microscope (Olympus, Shinjuku, Japan), and photographed with a Leica DFC 7000 T camera (1,920 × 1,440 pixel) attached to the microscope using the Leica Application Software V4.9 (Leica Microsystems CMS GmbH, Wetzlar, Germany). Stacked photographs were generated from series of photographs using CombineZP. The photographs were carefully adjusted for brightness and contrast and further assembled and labelled in CorelDraw 11 (Corel, Ottawa, Canada) .
The innervation pattern of the sensory structures in the tibia was documented by drawing (Leitz microscope with a drawing attachment; Leitz, Wetzlar, Germany). The schematic was redrawn digitally using CorelDraw 11.

Strauß J. (2023). Comparative Neuroanatomy of the Mechanosensory Subgenual Organ Complex in the Peruvian Stick Insect, Oreophoetes peruana. Brain, behavior and evolution, 98(1).

+ Open protocol

+ Expand

Microscopic Analysis of Insect Leg Innervation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Before microscopy of the tracing preparations, the posterior cuticle in the tibia of tracing preparations was removed with a piece of a blade. The presence of the posterior subgenual organ and its neuronal innervation (see section "Results") were checked to ensure that the complete sensory organs were present after opening the leg. The preparations were mounted on a microscopy slide and were in most cases viewed from the posterior side. Occasionally, legs were also documented from dorsal direction.
The legs were viewed on a microscopy slide under methyl salicylate (Merck, Darmstadt, Germany) with an Olympus BH-2 microscope (Olympus, Shinjuku, Japan). Digital photographs were acquired with a Leica DFC 7000 T camera (1,920 × 1,440 pixel) attached to the microscope via the Leica Application Software V4.9 (Leica Microsystems CMS GmbH, Wetzlar, Germany). Series of photographs were combined using CombineZP. Photographs were assembled into panels, adjusted for contrast and brightness, and labeled using CorelDraw 11 (Corel, Ottawa, Canada).
The innervation pattern of the tibia was drawn using a Leitz microscope combined with a drawing attachment (Leitz, Wetzlar, Germany), and digitally redrawn and labeled using CorelDraw 11.

Strauß J., Moritz‍ L., & Rühr P.T. (2021). The Subgenual Organ Complex in Stick Insects: Functional Morphology and Mechanical Coupling of a Complex Mechanosensory Organ. Frontiers in Ecology and Evolution, 9.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!