Qsg 7701

Normal hepatocyte QSG‐7701 and HCC cell lines HepG2, SK‐HEP‐1 and Huh7 were obtained from BeNa Culture Collection. All cells were authenticated by short tandem repeat profiling. The cells were cultured in high‐glucose DMEM medium (Invitrogen) added with 10% foetal bovine serum (FBS, Invitrogen) in a culture chamber with 5% CO₂ at 37°C. Cells were treated with 50 µmol/L α‐amanitin (Sigma‐Aldrich) for 0‐24 hours as indicated in this article.

HCC Cells (SMMC-7721, Hep3B, HCCLM3, HepG2, MHCC97H, Huh7, QSG-7701), and LO2 cells (as control cells) were bought from Bena Culture Collection (Kunshan, Jiangsu, China). They were cultured by RPMI-1640 media (with 10% fetal bovine serum). The cell culture condition was: 37 ˚C, 5% CO₂. The siRNAs (siRNA-control, siTNA-STAT1, siGOLM1-1, siGOLM1-2), miR-653 mimics and inhibitors were purchased from JiMa Biological corporation (Suzhou, Jiangsu, China). The overexpressing plasmids, including pcDNA3.1-STAT1, pcDNA3.1-ZFPM2-AS1, and pcDNA3.1- GOLM1, were Genetong Biological corporation (Xiamen, Fujian, China). The cell transfection was conducted using Lipofectamine 2000 reagent kits (GuangHua Biotech, Changsha, Hunan, China) in accordance with the kits’ protocols.