The protocol of western blot was shown before [18 (link)]. Briefly, total protein was extracted by RIPA buffer, and centrifuged at 16,000 × g for 20 min, the supernatant was taken for protein quantification. Then, 30 μg protein was used for sample loading, and transferred onto PVDF membrane, followed by incubation with HK2 antibody (ab104836, Abcam), YTHDF1 (ab220162, Abcam), METTL3 (ab195352, Abcam) and GAPDH (ab2735, Abcam) overnight at 4°C. The second day, the membrane was incubated with HRP-conjugated IgG at 37°C for 1 h. After washing with TBST, the blot was developed using ready-to-use ECL solution (Invitrogen).
Ab2735
Manufactured by Abcam
Ab2735 is a rabbit monoclonal antibody targeting Fibronectin. It is intended for use in immunohistochemistry and western blot applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ab2735
1
Quantification of RNA-related Proteins
2
Protein Extraction and Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total protein was extracted by RIPA buffer, and centrifuged at 16,000×g for 20min, the supernatant was taken for protein quanti cation. Then, 30µg protein was used for sample loading, and transferred onto PVDF membrane, followed by incubation with HK2 antibody (ab104836, Abcam), YTHDF1 (ab220162, Abcam), METTL3 (ab195352, Abcam) and GAPDH (ab2735, Abcam) overnight at 4 °C. The second day, the membrane was incubated with HRP-conjugated IgG at 37°C for 1h. After washing with TBST, the blot was developed using ready-to-use ECL solution (Invitrogen).
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