MDA-MB-231 cells were obtained from American Type Culture Collection (ATCC) and were cultured in low-glucose DMEM. MDA-MB-468 cells [15] (link) were obtained from Janet Price (MD Anderson, University of Texas, Houston, TX) and maintained in DMEM/F12 (1∶1). These two cell lines were cultured with 10% FBS and 1% penicillin/streptomycin/glutamine (Life Technologies). Immortalized non-malignant human breast epithelial MCF10A cell line was obtained from ATCC and maintained in DMEM/F12 media with 5% horse serum supplemented with 20 ng/ml EGF, 10 µg/ml insulin, 0.5 µg/ml hydrocortisone, 100 ng/ml Cholera toxin and 1% penicillin/streptomycin/glutamine. AGOH and AFOH were synthesized as described previously [16] (link), [17] (link). The relationship between these compounds and the natural isoprenoids is illustrated in Figure 1 .
Penicillin streptomycin glutamine (psg)
Manufactured by American Type Culture Collection
Penicillin/streptomycin/glutamine is a sterile, stabilized mixture of antibiotics and an amino acid. It is commonly used as a supplement in cell culture media to prevent bacterial contamination.
Automatically generated - may contain errors
Lab products found in correlation
Mcf 10a, by American Type Culture Collection (1 mentions)
Mda mb 231 cell, by American Type Culture Collection (1 mentions)
Penicillin streptomycin glutamine (psg), by Thermo Fisher Scientific (1 mentions)
Hek293 cell, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by American Type Culture Collection (1 mentions)
Penicillin, by American Type Culture Collection (1 mentions)
Streptomycin, by American Type Culture Collection (1 mentions)
L glutamine, by American Type Culture Collection (1 mentions)
2 protocols using penicillin streptomycin glutamine (psg)
1
Cultivation and Characterization of Breast Cancer Cell Lines
2
Heterologous Expression of Ion Channels
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All chemicals were sourced from Sigma (St. Louis, MO ,USA) and cell culture reagents were from Thermo Fisher Scientific (Waltham, MA, USA). HEK293 cells (ATCC CRL-1573.3) were cultured in DMEM supplemented with 10% fetal bovine serum (FBS), penicillin-streptomycin-glutamine (PSG, penicillin (100 units/ml)), streptomycin (100 µg/ml), and L-glutamine (290 µg/ml)). For expression of Schistosoma mansoni TRPM PZQ (Sm.TRPM PZQ ), a codon-optimized cDNA construct (Genscript, Piscataway, NJ, USA) was transiently transfected into HEK293 cells using Lipofectamine-2000. The sequence for Nematostella vectensis TRPM2 channel (Nv.TRPM2) was codon-optimized and synthesized from the NCBI Reference accession (XP_001622235.2). The construct encoding mPiezo1-IRES-eGFP was a gift from Ardem Patapoutian (Addgene plasmid #80925; http://n2t.net/addgene:80925; RRID:Addgene_80925).
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