Acetone, diethylether, magnesium sulfate, sodium hydroxide, dichloromethane, chlorobenzene, toluene, ethyl acetate, methanol, ethylbenzene, butylbenzene, hexylbenzene, phenyloctane, tetrahydrofuran (THF), chloroform, n-hexane were purchased from Nacalai Tesque (Kyoto, Japan), methyl pentafluorobenzoate (MPFB), diethyl amine, 3-aminopropyltrimethoxysilane (APTMS), d -( +)-trehalose dihydrate from Tokyo Chemical Industry (Tokyo, Japan), sodium azide, N-hydroxysuccinimide (NHS), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC), d -( +)-glucose, maltose monohydrate, sucrose from Wako Pure Chemical Industries (Osaka, Japan), C60-fullerene (C60), C70-fullerene (C70), dextran (Mw = 1,000) (glucose homopolymer) from Sigma-Aldrich Japan (Tokyo, Japan), 2-AB Dextran Calibration Ladder (2-AB labeled glucose homopolymer) from Waters (USA), respectively. Deionized water was obtained by a Milli-Q Direct-Q 3UV system (Merck Millipore, Tokyo, Japan). Silica monolithic capillaries (ULTRON HF-SIL, ULTRON HF-ODS) were purchased from Shinwa Chemical Industries Ltd. (Kyoto, Japan). Porous graphite carbon column (PGC column) was purchased from Thermo Fischer Scientific USA).
1 ethyl 3 3 dimethylaminopropyl carbodiimide
Manufactured by Fujifilm
Sourced in Japan
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide is a chemical compound commonly used as a coupling agent in biochemical research and laboratory experiments. Its core function is to facilitate the formation of amide bonds between carboxyl groups and amine groups in various biomolecules, such as proteins, peptides, and nucleic acids.
Automatically generated - may contain errors
Lab products found in correlation
Ethyl acetate, by Nacalai Tesque (1 mentions)
Tetrahydrofuran, by Nacalai Tesque (1 mentions)
Sodium hydroxide (naoh), by Nacalai Tesque (1 mentions)
Dichloromethane, by Nacalai Tesque (1 mentions)
Sucrose, by Fujifilm (1 mentions)
Sodium azide, by Fujifilm (1 mentions)
Methanol, by Nacalai Tesque (1 mentions)
Toluene, by Nacalai Tesque (1 mentions)
Maltose monohydrate, by Fujifilm (1 mentions)
D glucose, by Fujifilm (1 mentions)
N hydroxysuccinimide, by Fujifilm (1 mentions)
Diethylamine, by Tokyo Chemical Industry (1 mentions)
Magnesium sulfate, by Nacalai Tesque (1 mentions)
Milli q direct q 3uv system, by Merck Group (1 mentions)
3 aminopropyltrimethoxysilane, by Tokyo Chemical Industry (1 mentions)
N hexane, by Nacalai Tesque (1 mentions)
Acetone, by Nacalai Tesque (1 mentions)
Chloroform, by Nacalai Tesque (1 mentions)
Diethyl ether, by Nacalai Tesque (1 mentions)
Neurobasal medium, by Thermo Fisher Scientific (1 mentions)
2 protocols using 1 ethyl 3 3 dimethylaminopropyl carbodiimide
1
Comprehensive Analytical Reagent Procurement
2
Gelatin Sponge Preparation and Functionalization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The gelatin (GE) sponge was prepared as described previously (Ajioka et al., 2015) with some modification. Fifty microliters of 3% GE beMatrix Gelatin LS-H (Nitta Gelatin, Osaka, Japan) was added to each well of a 384-well plate and frozen at -20 C. The frozen GE samples were then lyophilized at 25 C, with centrifugation at 400 rpm (VC-96W; Taitek, Saitama, Japan). The freeze-dried GE samples were then crosslinked with 25 mM 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (Wako) in 90% acetone at room temperature overnight. After washing five times with double distilled water, the GE sponge was incubated in Neurobasal medium (GIBCO) for 3 h. Then, GE sponge was cut into blocks (1.2 3 1.2 3 1.2 mm 3 ) and conjugated with 10 mg/mL N-cadherin-Fc (IgG-Fc fused with the extracellular domain of mouse N-cadherin) or Fc solutions (Yue et al., 2010) for 24 h at 4 C.
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