Pp 3 mcpd d 5

The following analytical standards were purchased from Toronto Research Chemicals (North York, ON, Canada) at a purity > 98%: 3-MCPD-1,2-dipalmitoyl ester (PP-3-MCPD) and 3-MCPD-1,2-dipalmitoyl-d 5 ester (PP-3-MCPD-d 5 ). The stock solutions were prepared at a concentration of 0.5 mg ml -1 by dissolving the standards in tetrahydrofuran.

Analytical standards of 3-MCPD-1,2-dipalmitoyl ester (PP-3-MCPD) and 3-MCPD-1,2-dipalmitoyl-d 5 ester (PP-3-MCPD-d 5 ) at a purity higher than 98% were purchased from Toronto Research Chemicals (North York, ON, Canada). Stock solutions at a concentration of 0.5 mg ml -1 were prepared by dissolving the standards in tetrahydrofuran (THF, analytical grade).
Other chemicals used in the experiments included: acid phenylboronic (PBA, 97% purity), supplied by Sigma-Aldrich Corp. (Steinheim, Germany); methanol (HPLC grade), acquired from Tedia Company Inc. (Fairfield, OH, USA); tetrahydrofuran (HPLC grade), supplied by JT Baker (USA); hexane, acetone, petroleum ether, diethyl ether, concentrated hydrochloric acid, sulfuric acid, sodium bicarbonate, sodium sulfate, and ammonium sulfate (analytical grade), purchased from Labsynth (Diadema, SP, Brazil); silver nitrate (99.0-100.5% purity) and potassium chromate (≥99.5% purity), acquired from Merck (Darmstadt, Germany); and ultrapure water, obtained from a Milli-Q Plus system (Millipore, Bedforte, MA, USA).