2 3 dioleoyloxy propyl trimethylammonium dotap

Manufactured by Avanti Polar Lipids

Sourced in United States

DOTAP is a cationic lipid commonly used in liposome formulations for nucleic acid delivery. It is a synthetic lipid with a positively charged headgroup and two oleic acid chains. DOTAP is often used to facilitate the transfection of cells with plasmid DNA, mRNA, or other nucleic acid-based molecules.

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Lab products found in correlation

Phosphatase inhibitor cocktail, by Merck Group (1 mentions) Anti mouse pd 1 antibody clone rmp1 14, by BioXCell (1 mentions) 1 1 dioctadecyl 3 3 3 3 tetramethylindotricarbocyanine iodide dir, by Merck Group (1 mentions) Tryptone lp0042, by Thermo Fisher Scientific (1 mentions) Yeast extract lp0021, by Thermo Fisher Scientific (1 mentions) Hoechst 33258, by Merck Group (1 mentions) 1 2 dioleoyl sn glycero 3 phosphate dopa, by Avanti Polar Lipids (1 mentions) Gemcitabine, by Eli Lilly (1 mentions) Phosphatidylglycerol, by Merck Group (1 mentions) Oleic acid, by Merck Group (1 mentions) Human tf iron free, by Merck Group (1 mentions) N hydroxysuccinimide nhs, by Merck Group (1 mentions) Hl 60, by American Type Culture Collection (1 mentions)

2 protocols using 2 3 dioleoyloxy propyl trimethylammonium dotap

Formulation and Characterization of DOTAP/DOPA Lipoplex

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(2,3-dioleoyloxy-propyl)-trimethylammonium (DOTAP) and 1,2-dioleoyl-sn-glycero-3-phosphate (DOPA) were obtained from Avanti Polar Lipids (Alabaster, AL, USA). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (ammonium salt) (DSPE-PEG2000) was obtained from Xi’an Ruixi biotech (Xi’an, China). FHK peptide (FHKHKSPALSPV) was synthesized by China Peptides Co., Ltd (Shanghai, China). Low molecular weight protamine (LMWP), IGEPAL® CO-520, cholesterol, 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide (DiR), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT), 4,6-Diamidino-2-phenylindole (DAPI), Hoechst 33258 and phosphatase inhibitor cocktails were provided by Sigma-Aldrich (St. Louis, MO, USA). α-M were purchased from Biopurify Co., Ltd (Chengdu, China). Collagenase type IV (40510ES60), hyaluronidase (20426ES60), DNase I (10608ES25) and Agar (70101ES76) were purchased from Shanghai yeasen biotech (Shanghai, China). Tryptone (LP0042) and Yeast extract (LP0021) were obtained from OXOID. Gemcitabine was obtained from Eli Lilly. Anti-mouse PD-1 antibody (clone: RMP1-14) and IgG isotype (MPC-11, BP0086) are acquired from BioXcell. All the other chemical reagents and solvents were acquired from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China) unless specified.

Huang Y., Chen Y., Zhou S., Chen L., Wang J., Pei Y., Xu M., Feng J., Jiang T., Liang K., Liu S., Song Q., Jiang G., Gu X., Zhang Q., Gao X, & Chen J. (2020). Dual-mechanism based CTLs infiltration enhancement initiated by Nano-sapper potentiates immunotherapy against immune-excluded tumors. Nature Communications, 11, 622.

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Preparation and Culture of HL60 Cells

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Drn, Lut, oleic acid (OA), phosphatidylglycerol, human Tf (iron-free), and N-hydroxysuccinimide (NHS) were purchased from Sigma (St Louis, MO, USA) and (2,3-dioleoyloxy-propyl)-trimethylammonium (DOTAP) was provided by Avanti Polar Lipids (Birmingham, AL, USA). PEG-COOH (NH₂-PEG-COOH) and DSPE-PEG-COOH were obtained from Ponsure Biological (Shanghai, China). The human leukocyte cell line HL60 was obtained from the American Type Culture Collection (Manassas, VA) and cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS at 37°C in a 5% CO₂ incubator.

Zhu Y., Zhang W, & Chen J. (2023). Binary Nanodrug-Delivery System Designed for Leukemia Therapy: Aptamer- and Transferrin-Codecorated Daunorubicin- and Luteolin-Coloaded Nanoparticles. Drug Design, Development and Therapy, 17, 1-13.

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