Nc nitrocellulose membranes

Manufactured by Cytiva

NC Nitrocellulose Membranes are a type of laboratory equipment used for various applications in life science research. These membranes are made of nitrocellulose, a material that has a high affinity for proteins and other biomolecules. The core function of NC Nitrocellulose Membranes is to facilitate the immobilization and detection of these molecules in techniques such as Western blotting, dot blotting, and membrane-based assays.

Automatically generated - may contain errors

Lab products found in correlation

Mgatp, by Merck Group (1 mentions) Benzonase nuclease hc, by Merck Group (1 mentions) Insulin, by Merck Group (1 mentions) Pierce cell surface protein isolation kit, by Thermo Fisher Scientific (1 mentions) Streptavidin hrp, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Nitrocellulose (NC) membranes Nitrocellulose membranes NC membrane Nitrocellulose

2 protocols using nc nitrocellulose membranes

Hydrolysis of Styrene-Maleic Anhydride Copolymer

Check if the same lab product or an alternative is used in the 5 most similar protocols

SMA2000 (a copolymer of styrene and maleic anhydride) was supplied by Cray Valley and hydrolysed to form SMA using the protocol described by^{37 (link)}. Insulin, Zinc, Human, Recombinant, P. pastoris (#407709), Adenosine 5’-triphosphate magnesium salt from bacterial source (Mg-ATP) (#A9187) and ECLTM Select Western Blotting Detection Reagent (#GERPN2235) was supplied by Sigma Aldrich. NC Nitrocellulose Membranes (#15249794, Cytiva Amersham™ Protran™). Gibco™ 10 × concentrated Dulbecco’s phosphate-buffered saline (#14200-067), HaltTM Protease & Phosphatase Single-use Inhibitor Cocktail (100x) (#78442) and HaltTM Protease Single-use Inhibitor Cocktail (100x) (#78430) were supplied by ThermoFisher Scientific. Benzonase® Nuclease HC, Purity > 90% (#71205, Millipore). Calf intestine alkaline phosphatase Quick CIP (#M0525, New England BioLabs). Unless otherwise stated all chemical reagents and cell culture media and supplement were from Sigma Aldrich.

Morrison K.A., Wood L., Edler K.J., Doutch J., Price G.J., Koumanov F, & Whitley P. (2022). Membrane extraction with styrene-maleic acid copolymer results in insulin receptor autophosphorylation in the absence of ligand. Scientific Reports, 12, 3532.

+ Open protocol

+ Expand

Hydrolysis of Styrene-Maleic Anhydride Copolymer

Check if the same lab product or an alternative is used in the 5 most similar protocols

SMA 2000 (a copolymer of styrene and maleic anhydride) was supplied by Cray Valley and hydrolyzed to form SMA using the protocol described by Hall et al. (2018 (link)). NC Nitrocellulose Membranes (#15249794, Cytiva Amersham™ Protran™), Gibco™ 10× concentrated Dulbecco’s phosphate-buffered saline (#14200–067), Halt™ Protease Single-Use Inhibitor Cocktail (100x) (#78430), Pierce™ Cell Surface Protein Isolation Kit (#89881), and Streptavidin-HRP (#10015714) were supplied by ThermoFisher Scientific. All other chemical reagents, cell culture media, and supplements were from Sigma Aldrich.

Morrison K.A., Heesom K.J., Edler K.J., Doutch J., Price G.J., Koumanov F, & Whitley P. (2021). Development of Methodology to Investigate the Surface SMALPome of Mammalian Cells. Frontiers in Molecular Biosciences, 8, 780033.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!