CBC was measured by a Sysmex XN-1000 (Japan (19723); BM Egypt Company). Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were evaluated using the kinetic UV optimized method IFCC (LTEC Kit, England) [23 (link),24 (link)]. Serum albumin was quantified utilizing the technique with enhanced specificity of bromocresol green colorimetric assay (Diamond Diagnostics Kit, Germany) [25 (link)]. Electrochemiluminescence immunoassay (ECLA) on COBAS immunoassay analyzer was employed for assessing HCV antibody (anti-HCV) [26 (link)], while HBsAg was detected using Sorin Biomedica Co. kit (Italy) [27 (link)]. AFP was measured by enzyme-linked immunosorbent assay (ELISA) (IMMULITE 1000 system by a kit provided by Siemens Medical Solutions Diagnostics, USA) [28 ]. Moreover, the ELISA technique was used to estimate the serum level of TGF-β1 (Human TGF-β ELISA Kit, SunRed, China) [29 (link)]. HCV RNA was detected by RT-PCR using COBAS TaqMan HCV quantitative test, version 2.0 (Roche Molecular Systems, Inc., Branchburg, NJ, USA) [30 (link)].
Cobas taqman hcv quantitative test version 2
Manufactured by Roche
Sourced in United States
The COBAS TaqMan HCV quantitative test, version 2.0, is a laboratory diagnostic tool developed by Roche. It is designed to quantitatively detect and measure the hepatitis C virus (HCV) RNA levels in patient samples. The test utilizes real-time PCR technology to provide accurate and reliable results for the management of HCV infection.
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2 protocols using cobas taqman hcv quantitative test version 2
1
Comprehensive Blood Analysis for Hepatitis Diagnosis
2
Quantitative Detection of HCV by RT-PCR
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Anti-HCV antibodies were detected using an immunoradiometric assay (Shin Jin Medics, Inc., Goyang, Korea) based on recombinant proteins from the HCV genome, which showed a sensitivity of 98.5% and a specificity of 99.7%. Serum HCV RNA titers were quantitatively determined by real-time polymerase chain reaction (PCR) using COBAS TaqMan HCV quantitative test, version 2.0 (Roche Molecular Systems, Inc., Branchburg, NJ, USA) with a linear range from 43 to 69,000,000 IU/mL. In the present study, we classified patients into two groups with high or low viral load based on the serum HCV RNA level at study entry. The cut-off log titer of HCV RNA levels was 6: (1) high viral load > 6 log titer of HCV RNA IU/mL and (2) low viral load ≦ 6 log titer of HCV RNA IU/mL. HCV genotyping was performed by reverse transcription PCR and sequencing analysis using ABI PRISM 3130 Genetic Analyzer (Life Technologies, Carlsbad, CA, USA).
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