The mouse metastatic mammary 4T1 tumor cell line (Cat# CRL-2539) and human MDA-MB-231 breast adenocarcinoma cell line (Cat# HTB-26) were purchased from the American Type Culture Collection (ATCC). The MDA-MB-231-Luc-D3H2LN Bioware (D3H2LN) cell line [49 (link)] was purchased from PerkinElmer (Cat# 119369). The mouse mammary tumor MMTV-Myc cell line has been previously reported [25 (link), 50 (link)]. Cell lines were authenticated by short tandem repeat (STR) profiling in accordance with the standard ASN-0002-2011 in April 2015 and March 2016 (DDC Medical). 4T1 cells were maintained in RPMI supplemented with 10% fetal bovine serum (FBS) and 1% Antibiotic-Antimycotic solution (Invitrogen). The MDA-MB-231 cell line was maintained in DMEM supplemented with 10% FBS, 1% GlutaMAX (Invitrogen), 10 mM HEPES, 1 mM sodium pyruvate, non-essential amino acids and 1% Antibiotic-Antimycotic solution. MMTV-Myc cells were cultured in DMEM/F12 medium supplemented with 5% FBS, 1% GlutaMAX, 10 mM HEPES, and 1% Antibiotic-Antimycotic solution.
Human mda mb 231 breast adenocarcinoma cell line
Manufactured by American Type Culture Collection
Sourced in United States
The Human MDA-MB-231 breast adenocarcinoma cell line is a well-established in vitro model derived from a metastatic breast cancer tumor. It serves as a tool for researchers to study various aspects of breast cancer biology and drug development.
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Lab products found in correlation
Glutamax, by Thermo Fisher Scientific (3 mentions)
Antibiotic antimycotic solution, by Thermo Fisher Scientific (3 mentions)
Mouse metastatic mammary 4t1 tumor cell line, by American Type Culture Collection (3 mentions)
Mda mb 231 cell line, by Thermo Fisher Scientific (2 mentions)
Mda mb 231 luc d3h2ln bioware d3h2ln cell line, by PerkinElmer (2 mentions)
Turbofect, by Thermo Fisher Scientific (2 mentions)
Ivermectin, by Merck Group (1 mentions)
Mda mb 231, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin solution, by Corning (1 mentions)
4t1 murine breast cancer cell line, by American Type Culture Collection (1 mentions)
Sk ov 3 human ovarian adenocarcinoma cell line, by American Type Culture Collection (1 mentions)
Hela human cervical adenocarcinoma cell line, by American Type Culture Collection (1 mentions)
Mcf 7 human breast adenocarcinoma cell line, by American Type Culture Collection (1 mentions)
5 protocols using human mda mb 231 breast adenocarcinoma cell line
1
Metastatic Breast Cancer Cell Culture
2
Breast Cancer Cell Lines and Drug Evaluation
3
Characterization of Metastatic Breast Cancer Cell Lines
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The mouse metastatic mammary 4T1 tumor cell line (Cat# CRL-2539) and human MDA-MB-231 breast adenocarcinoma cell line (Cat# HTB-26) were purchased from the American Type Culture Collection (ATCC). The mouse mammary tumor MMTV-Myc cell line has been previously reported [62 (link), 63 (link)]. Cell lines were authenticated by short tandem repeat (STR) profiling in accordance with the standard ASN-0002-2011 in April 2015 (DDC Medical). 4T1 cells were maintained in RPMI supplemented with 10% fetal bovine serum (FBS) and 1% Antibiotic-Antimycotic solution (Invitrogen). The MDA-MB-231 cell line was maintained in DMEM supplemented with 10% FBS, 1% GlutaMAX (Invitrogen), 10mM HEPES, 1mM sodium pyruvate, non-essential amino acids and 1% Antibiotic-Antimycotic solution. MMTV-Myc cells were cultured in DMEM/F12 medium supplemented with 5% FBS, 1% GlutaMAX, 10mM HEPES, and 1% Antibiotic-Antimycotic solution. Stable knockdown of PF1 in MDA-MB-231 was performed with pLKO-PF1 shRNA (clone# TRCN0000015704), which was a kind gift from the laboratory of Gregory David (NYU School of Medicine, NY, USA) [44 (link)]. A second pLKO-PF1 shRNA construct (clone# TRCN0000084422, Sigma Aldrich) was used for stable knockdown in mouse 4T1 cells. Stable transfections were performed with 1 μg of DNA using TurboFect (ThermoScientific) according to the manufacturer's recommendations.
4
Culturing Breast Cancer Cell Lines
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The authenticated 4T1 murine breast cancer cell line, luciferase-expressing 4T1-Luc cell line and MDA-MB-231 human breast adenocarcinoma cell line were obtained from American Type Culture Collection (Rockville, USA). All cell lines were cultured at 37°C with 5% CO2 in DMEM supplemented with 10% Fetal Bovine Serum (FBS) (Gibco, USA), and 1% Penicillin-Streptomycin Solution (Corning, NY, USA).
5
Intracellular ROS Detection by HSN in Cell Lines
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4T1 murine mammary carcinoma cell line, MCF-7 human breast adenocarcinoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH/3T3 murine fibroblast cell line, NDF normal human dermal fibroblast cell line, MDA-MB-231 human breast adenocarcinoma cell line, PC12 rat pheochromocytoma cell line, HeLa human cervical adenocarcinoma cell line, and SKOV3 human ovarian adenocarcinoma cell line (purchased from American Type Culture Collection, ATCC) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% antibiotics (penicillin and streptomycin) and placed in a humid CO2 incubator providing an atmosphere containing 5% CO2 at 37 °C.
To detect the intracellular ROS aroused by HSN, cells were seeded in confocal cell culture dishes at a density of 1 × 104 cells per dish. After culture in incubator for 12 h, HSN (final concentration: [pTBCB] = 50 µg mL−1) or PBS were added to the cells and incubated with cells for 24 h. Thereafter, cells were gently washed three times with fresh PBS to remove excess nanoparticles, and then fixed with 4% paraformaldehyde. After fixation, cells were successively stained with DAPI and DCF-DA. Then cells were imaged under a LSM800 confocal microscope.
To detect the intracellular ROS aroused by HSN, cells were seeded in confocal cell culture dishes at a density of 1 × 104 cells per dish. After culture in incubator for 12 h, HSN (final concentration: [pTBCB] = 50 µg mL−1) or PBS were added to the cells and incubated with cells for 24 h. Thereafter, cells were gently washed three times with fresh PBS to remove excess nanoparticles, and then fixed with 4% paraformaldehyde. After fixation, cells were successively stained with DAPI and DCF-DA. Then cells were imaged under a LSM800 confocal microscope.
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