Dnmdp

The human GIST882 cell line [49 (link)] was kindly provided by Dr. Jonathan A. Fletcher, Harvard Medical School, Boston, MA, USA. Cells were cultured at 37°C in DMEM (GIBCO, CA, USA) supplemented with 10% FBS, 2% penicillin/streptomycin. The human GIST48 cell line [18 (link)] was kindly provided by Dr. Ronald DeMatteo, Perelman School of Medicine, Philadelphia, PA, USA. Cells were cultured at 37°C in RPMI-1640 (GIBCO, CA, USA) supplemented with 10% FBS, 2 mM L-glutamine, 50 U/mL penicillin-streptomycin, 0.1% 2- mercaptoethanol, and 10 mM Hepes. Cilostazol, forskolin and verteporfin were purchased from Sigma, St. Louis, MO, USA, imatinib was purchased from LC Laboratories, Woburn, MA, USA, and DNMDP was purchased from Aobious Inc, Gloucester, MA, USA.

The human GIST cell line GIST882 was kindly provided by Dr. Jonathan A. Fletcher, Harvard Medical School, Boston, MA, USA. Cells were cultured at 37°C in DMEM (GIBCO, CA, USA) supplemented with 10% FBS, 2% penicillin/streptomycin. HeLa cells were cultured at 37°C in DMEM (GIBCO, CA, USA) supplemented with 10% FBS, 2% penicillin/streptomycin. HEK293T were cultured at 37°C in DMEM + GlutaMAX–I (GIBCO, CA, USA) supplemented with 10% FBS, 2% penicillin/streptomycin. Cilostazol, U0126 and forskolin were purchased from Sigma, St. Louis, MO, USA, STI-571 was purchased from LC Laboratories, Woburn, MA, USA, DNMDP was purchased from Aobious Inc, Gloucester, MA, USA and Akti was purchased from Merck, Darmstadt, Germany.