Uplc ms grade water

Ifosfamide (IFO) and methotrexate (MTX) standards were European Pharmacopeia Reference Standards. Cyclophosphamide (CP) and deuterated CP (CP-d4) were purchased from Santa Cruz Biotechnology, Inc. (Dallas, Texas, USA). 5-fluorouracil (5-FU), 5-fluorouracil-2-13C, 15N2 (5-FU13C15N2), deuterated MTX (MTX-d3) as well as ammonium formate (AF) were purchased from Sigma-aldrich (Saint Louis, Missouri, USA). Formic acid (FA) for LC-MS was purchased from Fluka (Honeywell International Inc., New Jersey, USA). Ammonia was purchased from Chem-Lab Analytical (Zedelgem, Belgium) and UPLC-MS grade water from Biosolve (Valkenswaard, The Netherlands). LC-MS grade methanol (MeOH) from J.T. Baker and HPLC-MS grade acetonitrile (ACN) were purchased from VWR International (Radnor, Pennsylvania, USA). A rotator, model reax 2 from Heidolph, Schwabach, Germany was used.

UPLC/MS grade water, methanol and formic acid (>99.98%) were purchased from Biosolve Chimie, Dieuze, France; deuterium (99.8% D), dithiotreitol (98%), (E)-2-hexenal (98%), 2-hexyn-1-ol (≥97%), Boc-Cys-OH (≥98.5%), (Boc-Cys-OH)₂ (≥98%), ethanol-OD (≥99%), hydrochloric acid (37%), L-glutathione reduced (98%), Lindlar catalyst, manganese(IV) oxide (≥99%), mesityl oxide (≥90%), mesityl oxide d₁₀ (>98% D), triethylamine (≥99%) were purchased from Sigma-Aldrich, St Quentin Fallavier, France, and H-Cys-Gly-OH (98%), H-Glu(Cys-OH)-OH (98%)from Bachem, Bubendorf, Suisse; triethylsilane (≥98%) was obtained from TCI Europe, Zwijndrecht, Belgium; while di-sodium hydrogen phosphate (≥99%), sodium carbonate (≥99%), sodium dihydrogen phosphate dihydrate (≥99%)and trifluoroacetic acid (≥99.9%) were purchased from Carl Roth, Karlsruhe, Germany.