Gentamycin sulfate

BB was provided by Guizhou AiYuan Ecological Medicine Development Ltd. (Guizhou, China); TTO was purchased from Aladdin (Shanghai, China); Gentamycin sulfate and the bacterial growth media including LB Broth were obtained from Solarbio (Beijing, China). Complete Freund’s Adjuvant (CFA) was purchased from Sigma (St. Louis, MO, USA). P. aeruginosa, E. coli, and S. aureus were contributed by Guizhou Medical University. SD rats were provided by Guizhou University of Traditional Chinese Medicine.

All surgeries were conducted under aseptic conditions. After deep anesthetization with 4% inhaled isoflurane (R510-22, RWD, Shenzhen, China), the skin on the dorsal side of the spine was shaved and disinfected before surgery. A 1-cm midline longitudinal incision was made centered on T10 through the skin and muscle covering the T10 vertebrae. The spinal cord was exposed by a T10 vertebrae dorsal laminectomy. The NYU Impactor Model III (W.M.Keck, USA) was used to establish the moderate SCI contusion model by a 10 g rod with a height of 25 mm. The muscles and skin were sutured layer by layer. Sham group animals received a T10 laminectomy only. Manual bladder emptying was performed twice a day until the rats were able to urinate by themselves. Antibiotics (Gentamycin sulfate, Solarbio, G8170, Beijing, China) were administrated twice a day in the first 3 days post-injury (dpi).

Gentamycin Sulfate was purchased from Solarbio (Beijing, China). Phosphatidylcholine and Hydrogen tetrachloroaurate (III) were purchased from Aladdin (Shanghai, China). Citric acid monohydrate was purchased from Sinopharm Chemical Reagent Co., Ltd. All reagents were of analytical grade and used as received without further purifying.
Listeria monocytogenes (ATCC 19114), Staphylococcus aureus (ATCC 29213), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (PAO1) and Salmonella typhimurium (SL1344) were generous gifts received from Prof. Xia (College of Food Science and Engineering, Northwest A&F University).