The human Cytokine Array C5 and the human Growth Factor Array (RayBiotech, Peachtree Corners, GA, USA) were used to characterize media conditioned by mononuclear cells. For the assay, THP-1 cells or PBMCs (5 × 105 cells/mL) were cultured for 48 h at 37 °C (5% CO2, humidified atmosphere). Conditioned media were obtained after centrifugation at 1000× g for 10 min. The array was performed according to the manufacturer’s instructions. Chemiluminescent signals were detected with the Chemocam. Signal intensities, quantified using the ImageJ software, were normalized to the internal controls.
Human growth factor array
Manufactured by RayBiotech
Sourced in United States
The Human Growth Factor Array is a multiplex assay designed to quantitatively measure the levels of multiple human growth factors in a single sample. The array provides a comprehensive analysis of growth factor expression, enabling researchers to gain valuable insights into cellular signaling and growth-related processes.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using human growth factor array
1
Profiling Cytokine and Growth Factor Secretome
2
Meniscus Extracellular Matrix Protein Profiling
3
Extracellular Matrix Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Samples of native tendon and cartilage ECMs, and their corresponding urea-extracted and pepsin-digested extracts, were suspended in TM buffer (Total Protein Extraction Kit, EMD Millipore); 30 μg total protein was mixed with SDS loading buffer and reducing agent (NuPAGE; Life Technologies, Carlsbad, CA, USA) and heated for 10 min at 70 °C. Protein was loaded into a pre-cast 10-well NuPAGE 4–12% Bis-Tris Minigel (Life Technologies) and separated by electrophoresis in MOPS running buffer for 50 min at constant 200 V. The gel was stained with SimplyBlue™ SafeStain (Life Technologies) for 1 h, washed several times in water, and photographed using a CCD camera gel imaging system (FOTODYNE, Hartland, WI, USA).
Additionally, the growth factor contents of the soluble ECM preparations were determined using a Human Growth Factor Array (RayBiotech, Norcross, GA, USA) according to the manufacturer’s instructions.
Additionally, the growth factor contents of the soluble ECM preparations were determined using a Human Growth Factor Array (RayBiotech, Norcross, GA, USA) according to the manufacturer’s instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!