The method was presented in the previous study53 (link). In short, one hour after oral administration of D-xylose on day 12 of the experiment, blood samples were collected from the anterior vena cava into heparinized vacuum tubes (Becton–Dickinson Vacutainer System, Franklin Lake, NJ, USA) and then centrifuged at 3000 rpm for 10 min at 4 ℃ to obtain plasma. Plasma biochemical parameters were measured according to the manufacturer’s instructions by a Hitachi 7060 Automatic Biochemical Analyzer (Hitachi, Japan)75 (link). Blood cell counts were performed on the Siemens ADVIA 2120i Hematology Analyzer (Siemens Healthcare Diagnostics, Deerfield, Illinois, USA).
Advia 2120i hematology analyzer
Manufactured by Siemens
Sourced in United States, Germany
The ADVIA 2120i is a hematology analyzer designed to perform complete blood count (CBC) and differential analysis. It is capable of analyzing a wide range of blood parameters, including red blood cells, white blood cells, and platelets. The ADVIA 2120i utilizes advanced flow cytometry and colorimetric technologies to provide accurate and reliable results.
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Lab products found in correlation
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Architect c8000 analyzer, by Abbott (2 mentions)
Architect c16000, by Abbott (2 mentions)
7060 automatic biochemical analyzer, by Hitachi (1 mentions)
Vacutainer system, by BD (1 mentions)
Rx daytona clinical chemistry analyzer, by Randox (1 mentions)
Healthcare diagnostics kits, by Siemens (1 mentions)
Variant 2 hemoglobin testing system, by Bio-Rad (1 mentions)
Tba 120 fr chemistry analyzer, by Toshiba (1 mentions)
200fr neo chemistry analyzer, by Toshiba (1 mentions)
Automated biochemical technique, by Siemens (1 mentions)
Cs 5100, by Sysmex (1 mentions)
Au680 clinical chemistry analyzer, by Beckman Coulter (1 mentions)
Dimension vista 1500, by Siemens (1 mentions)
20 protocols using advia 2120i hematology analyzer
1
Plasma and Blood Cell Analysis Protocol
2
Red Blood Cell Quality Assessment
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The parameters used to assess the quality of the RCC, and WB were derived from the list presented in the Guidelines for Blood Transfusion Services 8th Edition,13 Section 8.2. Full details of the assays are presented in Appendix S1, which were conducted in accordance with published methodology. Hematology was performed using a standard laboratory analyzer (Siemens Advia 2120i Hematology Analyzer, Siemens Healthcare GmbH, Germany). Clinical chemistry assays (glucose, lactate, sodium, potassium, and hemoglobin) were performed with a Randox RX Daytona+ clinical chemistry analyzer, and hemolysis determined using a published formula.14 , 15 RBC microparticles were determined using flow cytometry16 , 17 and ATP and 2,3 DPG using published assays.18 Clotting factor and coagulation analyses were performed with an ACL TOP 300 CTS (Werfen, UK).
3
Comprehensive Blood Analysis Protocol
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After termination, approximately 1.5 mL of blood was analyzed for red blood cell, hematocrit, hemoglobin, mean corpuscular hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin concentration, reticulocyte, platelet, and white blood cell levels with an ADVIA 2120i hematology analyzer (Siemens, USA). Activated partial thromboplastin time and prothrombin time were analyzed with an ACL 9000 coagulation analyzer (Instrumentation Laboratory, Italy).
4
Veterinary Hematology Standardized Protocols
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Blood samples were analyzed in the Hematology Laboratory at the OSUVMC. Complete blood counts were determined using an Advia 2120i Hematology Analyzer (Siemens Healthineers, Plymouth, Massachusetts). For differential cell counts, blood films were stained with Wright‐Giemsa using a Harleco Midas II Automated Stainer (Millipore Sigma, St. Louis, Missouri). All assays were performed according to manufacturer's instructions using standardized protocols validated for veterinary use. Quarterly quality assurance testing, which includes peer review of blood films, is performed as part of a program administered by the Veterinary Laboratory Association. Commercial controls and calibrators are regularly evaluated.
5
Comprehensive Blood Biomarker Analysis
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General blood tests were performed by using a Siemens Advia 2120i Hematology Analyzer. For the biochemical analysis of serum components (urea, creatinine, uric acid, parathormone, glucose, cholesterol, triglycerides, calcium, phosphorus, potassium, sodium, chlorine, magnesium, iron, ferritin, total iron-binding capacity, proteins, albumin, b2-microglobulin, serum glutamyl oxalate transaminase, serum glutamyl pyruvate transaminase, gamma-glutamyl transferase, alkaline phosphatase, total bilirubin, indirect bilirubin, direct bilirubin, creatine phosphokinase, amylase, lactate dehydrogenase, and vitamin-D) the automatic Clinical Chemistry Analyzer ARCHITECT C16000 (Abbott, Illinois, IL, USA) was used. hs-CRP levels were determined with a commercial kit [Abbott Laboratories, Hellas (Greece)] in the Architect C8000 analyzer. Plasma free hemoglobin (fHb) was calculated by the method of Harboe [5 (link)].
6
Hematological and Clotting Evaluation
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Animals were fasted for 16 h prior to blood sample collection. Blood (∼1.5 mL) was collected from the abdominal vena cava of all animals at terminal sacrifice whilst under deep isoflurane anesthesia. Blood samples were mixed with potassium salt of EDTA and assayed for parameters listed in Table 3 , using an ADVIA2120i hematology analyzer (Siemens, USA). Clotting potential was assessed by mixing ∼1.0 mL blood with 3.2 % sodium citrate solution prior to centrifugation at ∼3000 rpm for 10 min at room temperature. Plasma was collected and analyzed for prothrombin time using an ACL 9000 coagulation analyzer (Instrumentation Laboratory, Italy).
7
Comprehensive Blood Profile Analysis
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Blood samples (n = 202) were drawn via jugular venipuncture and immediately placed on ice in two collection tubes containing the anticoagulant EDTA (ethylenediaminetetraacetic acid). The first tube was centrifuged at 4000 rpm for 20 min to separate plasma, and duplicates of 1 mL were stored at − 80 °C until NMR analysis. The second tube was used for complete blood counts (CBC), where red blood cells (RBC), hematocrit (HTC), hemoglobin (HGB), white blood cells (WBC), and platelet (PTL) contents were acquired using a veterinary hematology analyzer (Advia 2120i hematology analyzer, Siemens Healthcare Diagnostics Inc., Tarrytown, NY, USA). In addition, microscopic differential counts of WBC were performed by one co-author (EB) under the supervision of one of the board-certified veterinary clinical pathologists in the College of Veterinary Medicine (CVM) Diagnostic Laboratory to assess the variability of neutrophils, eosinophils, basophils, monocytes, and lymphocytes.
8
Glucose Tolerance Test in Rats
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At the end of 30 days experimental period, rats were kept for overnight fasting. The next morning “0” h blood samples were collected for estimation of hematological parameters, glucose, total cholesterol, and triglycerides. Hematological parameters were analyzed using Siemens Health care Diagnostic Kits (Newark, USA) on Advia 2120i Hematology analyzer (Siemens, Germany). Plasma glucose, total cholesterol, and triglycerides were analyzed using Siemens Healthcare Diagnostics Kits on the auto-blood analyzer. Glucose solution at the dose of 2 g/kg body weight was administered to all the rats. Blood was drawn at the intervals of 30 min for up to 120 min, and plasma glucose levels were estimated. Two-hour postprandial glycemic load[10 (link)] and delta-glucose (ΔG) values[14 (link)] were calculated accordingly.
Animals were euthanized under CO2 anesthesia after completion of the experiment. Relevant organs were surgically removed, cleared of adventitious tissues, washed, and stored in −80°C Freezer (NUAIRE-2100, Ultraflow freezer, Plymouth, MN, USA) for further analysis.
Animals were euthanized under CO2 anesthesia after completion of the experiment. Relevant organs were surgically removed, cleared of adventitious tissues, washed, and stored in −80°C Freezer (NUAIRE-2100, Ultraflow freezer, Plymouth, MN, USA) for further analysis.
9
Post-Operative Bleeding: Retrospective Analysis
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A retrospective analytical study was conducted. We collected data from the electronic charts of those medical centre patients who were diagnosed with post-operative bleeding between October 2007 and October 2017. We used the International Classification of Diseases, 9th Revision, Clinical Modification diagnosis codes 998.11 'Hemorrhage complicating a procedure' and 28.7 'Control of hemorrhage after tonsillectomy and adenoidectomy' to identify the patients. The Advia 2120i Hematology Analyzer (Siemens, Tarrytown, New York, USA) was used in the laboratory to determine the haemoglobin and haematocrit levels.
10
Hematological Analysis of Treated Animals
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All surviving males on the day after nal dosing and females on LD 5 were humanely sacri ced with iso urane. Blood for clinical pathology were collected from the caudal vena cava from 5 randomly selected animals/sex/group. Animals for blood collection were fasted approximately 16 hours (overnight) prior to sacri ce. Blood for hematology was placed into tubes containing potassium salt of ethylenediaminetetraacetic acid (EDTA) and then analyzed with an ADVIA2120i hematology analyzer (Siemens, Germany) for the following parameters: total red blood cell count (RBC), mean corpuscular volume (MCV), hemoglobin (HGB), hematocrit (HCT), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular hemoglobin (MCH), platelet count (PLT), reticulocyte count, total white blood cell count (WBC) and WBC differential count (absolute and relative counts of neutrophils [NEU],
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