Gfp tag monoclonal antibody

The monoclonal antibody TER-119 conjugated to Alexa Fluor® 488, recognizes a glycophorin A-associated protein on the surface of the red blood cell^{41 (link)}. The SEP1 antiserum produced in rabbits has been described^{42 (link)}. GFP was visualized by GFP Tag Monoclonal Antibody from Invitrogen. Secondary antibodies were anti-rabbit (Cy-3, Jackson Immuno-Research) and anti-mouse (Alexa Fluor® 647, Invitrogen).

According to the demand of the experiment, the corresponding fraction was loaded on 10% SDS-PAGE or 3%–12% Bis-Tris Native-PAGE (Invitrogen). Gels were blotted onto a polyvinylidene difluoride membrane (Bio-Rad). The membrane was immunoprobed using rabbit polyclonal antisera against RbcL and beta subunit of ATP synthase (Agrisera) and then goat anti-rabbit horseradish peroxidase-conjugated secondary antibody (Agrisera) or by using GFP tag monoclonal antibody (Invitrogen) and then rabbit anti-mouse horseradish peroxidase-conjugated secondary antibody (Agrisera). Immunoreactive polypeptides were visualized by using the western ECL blotting substrate (Bio-Rad). Signal quantification was carried out using Fiji. For each experiment, at least three independent cell cultures were performed.