Fmoc asn trt oh

Manufactured by GL Biochem

Sourced in China

Fmoc-Asn(Trt)-OH is an amino acid building block used in solid-phase peptide synthesis. It consists of an asparagine residue protected with Fmoc and Trt groups, which are commonly used protecting groups in peptide chemistry.

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Lab products found in correlation

Fmoc phe oh, by GL Biochem (4 mentions) Fmoc his trt oh, by GL Biochem (3 mentions) Fmoc pro oh, by GL Biochem (3 mentions) Fmoc lys boc oh, by GL Biochem (3 mentions) Fmoc arg pbf oh, by GL Biochem (3 mentions) Fmoc ser tbu oh, by GL Biochem (2 mentions) Fmoc tyr tbu oh, by GL Biochem (2 mentions) Fmoc ile oh, by GL Biochem (2 mentions) Fmoc cys trt oh, by GL Biochem (2 mentions) Fmoc ala oh, by GL Biochem (2 mentions) Nonadecanoic acid, by Merck Group (1 mentions) Fmoc trp boc oh, by GL Biochem (1 mentions) N n diisopropylcarbodiimide, by Merck Group (1 mentions) N n diisopropylethylamine, by Merck Group (1 mentions) Fmoc leu, by GL Biochem (1 mentions) Fmoc gly, by GL Biochem (1 mentions) Matrix metalloproteinase 9 mmp 9, by Merck Group (1 mentions) Thioflavin t, by Merck Group (1 mentions) Trisodium citrate, by Merck Group (1 mentions) Silver nitrate agno3, by Merck Group (1 mentions)

4 protocols using fmoc asn trt oh

Peptide Synthesis and Characterization

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The conventional amino acids, Fmoc-Ala, Fmoc-Lys(Boc)-OH, Fmoc-Arg(Pbf)-OH, Fmoc-Gly, Fmoc-His(Trt)-OH, Fmoc-Leu, Fmoc-Ser(tBu)-OH, Fmoc-Thr(OtBu)-OH, Fmoc-Trp(Boc)-OH, Fmoc-Tyr(tBu)-OH, Fmoc-Asn(Trt)-OH, Fmoc-Ile-OH, Fmoc-Phe-OH, Fmoc-Cys(Trt)-OH, Fmoc-Pro-OH, were acquired from GL Biochem Ltd. (Shanghai, China). N,N′-diisopropylcarbodiimide (DIC), Oxyma pure, 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate (HATU), N,N-Diisopropylethylamine, triisopropylsilane (TIS), 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP), nonadecanoic acid (C19) were purchased from Sigma-Aldrich (Italy). Rink amide p-methylbenzhydrylamine (MBHA) resin, Fmoc-L-Lys(Mtt)-OH, piperidine, and trifluoroacetic acid (TFA) were purchased from Iris Biotech GmbH (Marktredwitz, Germany). Anhydrous solvents [N,N-dimethylformamide (DMF) and dichloromethane (DCM)], Doxo-EMCH, matrix metalloproteinase-9 (MMP-9) and dialysis tubing benzoylated, Nile Red, and Thioflavin T were purchased from Sigma-Aldrich (Milan, Italy). Silver nitrate (AgNO₃), and trisodium citrate were obtained from Sigma-Aldrich (Luzais, Saint-Quentin-Fallavier, France). Ultrapure water was produced using a Barnstead EASYpure RoDi system (Thermo Fisher Scientific, Villebon sur Yvette, France). All chemicals and reagents were of analytical grade and used as received.

Del Genio V., Falanga A., Allard-Vannier E., Hervé-Aubert K., Leone M., Bellavita R., Uzbekov R., Chourpa I, & Galdiero S. (2022). Design and Validation of Nanofibers Made of Self-Assembled Peptides to Become Multifunctional Stimuli-Sensitive Nanovectors of Anticancer Drug Doxorubicin. Pharmaceutics, 14(8), 1544.

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Peptide Synthesis and Cell Viability Assay

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Fmoc-Phe-OH, Fmoc-Pro-OH, Fmoc-His(Trt)-OH, Fmoc-Cys(Trt)-OH, Fmoc-Asn(Trt)-OH, Fmoc-Lys(Boc)-OH, Fmoc-Ala-OH, Rink amide-AM resin, 1-hydroxybenzotriazole, benzotriazole-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate, and N-methylmorpholine were obtained from GL Biochem (Shanghai, China). Dimethylformamide, methanol, dichloromethane, and n-hexane were obtained from Beijing Chemical Factory (Beijing, China). Pluronic® F-127 and DMSO were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dess–Martin periodinane was provided by Aladdin (Shanghai, China). DMEM medium was purchased from Thermo Fisher Scientific (Shanghai, China). Fetal bovine serum was purchased from Kang Yuan Biology (Tianjin, China). Trypsin and Hoechst 33258 nuclear dye were obtained from Solarbio (Beijing, China). Thiazolyl blue tetrazolium bromide (MTT) was purchased from Genview (Beijing, China). WST-1 kit and Lysotraker Green procured from Beyotime (Shanghai, China). Annexin V-FITC/PI apoptosis detection kit was purchased from BestBio (Shanghai, China).

Han W., Meng F., Gan H., Guo F., Ke J, & Wang L. (2021). Targeting self-assembled F127-peptide polymer with pH sensitivity for release of anticancer drugs. RSC Advances, 11(3), 1461-1471.

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Synthesis and Characterization of Hydrogelator 1

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The reagents such as N, N-diisopropylethylamine (DIPEA), O-benzotriazole-N,N,N′,N′-tetramethyluronium-hexafluorophosphate (HBTU), were purchased from ACROS Organics USA. All other amino acid derivatives including Fmoc-Phe-OH, Fmoc-His(Trt)-OH, Fmoc-Asn(Trt)-OH, Fmoc-Pro-OH, Fmoc-Arg(pbf)-OH were purchased from GL Biochem (Shanghai) Ltd. The synthesis of hydrogelator 1 is based on solid phase peptide synthesis (SPPS) and has been reported previously. The crude compound that acquired from SPPS is purified by HPLC and the overall yield of the synthesis is about 80%. ¹H-NMR and LC-MS spectra are used to characterize the product.

Li J., Du X., Powell D.J., Zhou R., Shi J., He H., Feng Z, & Xu B. (2018). Down-regulating Proteolysis to Enhance Anticancer Activity of Peptide Nanofibers. Chemistry, an Asian journal, 13(22), 3464-3468.

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Solid-Phase Peptide Synthesis Protocol

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PL-Wang resin (0.4 mmole/g) was obtained from Varian, Inc. (Shropshire, UK). Fmoc-Ser(tBu)-OH, Fmoc-Met-OH, Fmoc-Leu-OH, Fmoc-Ile-OH, Fmoc-Ala-OH, Fmoc-Lys(Boc)-OH, Fmoc-Asp(OtBu)-OH, Fmoc-Asn(trt)-OH, Fmoc-Glu(OtBu)-OH, Fmoc-Phe-OH, Fmoc-Gln(trt)-OH, Fmoc-Tyr(tBu)-OH, Fmoc-Arg(pbf)-OH, N,N′-diisopropylcarbodiimide (DIC), 4-dimethylaminopyridine (DMAP) and triisopropylsilane (TIS) were obtained from GL Biochem Ltd. (Shanghai, China). Fmoc-Val-OH, Fmoc-Gly-OH, Fmoc-His(trt)-OH and N,N,N′,N′-tetramethyl-uronium-hexafluoro-phosphate (HBTU) were obtained from CS Bio Co. (Menlo Park, USA). Dimethylformamide (DMF), dichloromethane (DCM) and dimethyl sulfoxide (DMSO) were obtained from Daejung Chemicals & Metals Co., Ltd. (Siheung, Korea). Anisole, piperidine and N,N-diisopropylethylamine (DIEA) were obtained from Sigma-Aldrich (St. Louis, USA). Trifluoroacetic acid (TFA) was obtained from Yakuri Pure Chemicals Co., Ltd. (Kyoto, Japan). Ether, Anhydrous was obtained from J.T. Baker (Center Valley, USA). Zoletil® (Virbac) and Rompun® (Bayer Parma) were obtained from SMP animal medicine (Suwon, Korea). Protease inhibitor cocktail was obtained from Roche Diagnostics (IN, USA). EDTA treated BD vacutainer® was obtained from Becton, Dickinson and Company (NJ, USA). Human Aβ42 Ultrasensitive ELISA kit was obtained from Invitrogen (CA, USA).

Cho S.M., Kim H.V., Lee S., Kim H.Y., Kim W., Kim T.S., Kim D.J, & Kim Y. (2014). Correlations of amyloid-β concentrations between CSF and plasma in acute Alzheimer mouse model. Scientific Reports, 4, 6777.

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