Sil 10avp auto injector

Manufactured by Shimadzu

Sourced in Japan

The SIL-10Avp is an auto-injector module designed for Shimadzu's HPLC and UHPLC systems. It automates the process of sample injection, providing consistent and reliable sample introduction. The core function of the SIL-10Avp is to automatically draw samples from vials or plates and inject them into the liquid chromatography system for analysis.

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Lab products found in correlation

Fcv 10avp flow channel selection valves, by Shimadzu (2 mentions) Cto 10a column oven, by Shimadzu (1 mentions)

2 protocols using sil 10avp auto injector

HPLC Fingerprinting of Herbal Extracts

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The HPLC apparatus used was composed of an SCL-10Avp system controller, an LC-10ATvp liquid chromatographic pump, an SPD-M10A diode array detector, an SIL-10Avp auto-injector, a CTO-10A column oven, FCV-10Avp flow-channel selection valves (Shimadzu, Tokyo, Japan), and an ERC-3415 degasser (ERC, Altegolfsheim, Regensburg, Germany). The stationary phase was comprised of a Purospher® STAR RP-18e reverse-phase column (4 mm i.d. × 250 mm, 5 μm, Merck, USA), and the mobile phase system included 0.05% trifluoroacetic acid: CH₃CN gradient elution (5 min, 90:10; 15 min, 75:25; 25 min, 55:45; 35 min, 40:60; and 45 min, 0:100). The flow rate was 1 mL/min, and the column oven temperature was maintained at 40 °C. The ultraviolet wavelength was set to 280 nm for detecting the fingerprint chromatograms of the 50% ethanol extracts of DHJST, WDH, SWT, TH and CC.

Tseng S.H., Lee C.J., Chen S.H., Chen C.H., Tsai P.W., Hsieh M.S., Chu J.S, & Wang C.C. (2022). Cinnamic aldehyde, an anti-inflammatory component in Du-Huo-Ji-Sheng-Tang, ameliorates arthritis in II collagenase and monosodium iodoacetate induced osteoarthritis rat models. Journal of Traditional and Complementary Medicine, 13(1), 51-61.

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Quantification of Myristicin and Safrole

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The HPLC apparatus included an SCL-10Avp system controller, an LC-10ATvp liquid chromatographic pump, an SPD-M10A diode array detector, an SIL-10Avp auto-injector, a CTO-10A column oven, FCV-10Avp flow-channel selection valves (Shimadzu, Tokyo, Japan), and an ERC-3415 degasser (ERC, Altegolfsheim, Regensburg, Germany). The stationary phase comprised a Purospher^® STAR RP-18e reverse-phase column (4 mm i.d. × 250 mm, 5 μm, Merck, Kenilworth, NJ, USA), and the mobile phase system included 0.05% trifluoroacetic acid : CH₃CN isocratic at a ratio of 40:60. The flow rate was 1 mL/min, and the column oven temperature was maintained at 40 °C. The ultraviolet wavelength was set to 220 nm to detect myristicin and safrole in MFO and MFE-M. The concentration range of myristicin and safrole was 0.78 to 1000 μg/mL.

Lee C.J., Huang C.W., Chen L.G, & Wang C.C. (2020). (+)-Erythro-Δ8′-7S,8R-dihydroxy-3,3′,5′-trimethoxy-8-O-4′-neolignan, an Anti-Acne Component in Degreasing Myristica fragrans Houtt. Molecules, 25(19), 4563.

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