1260 infinity gpc system

Manufactured by Agilent Technologies

The 1260 Infinity GPC system is a liquid chromatography instrument designed for gel permeation chromatography (GPC) analysis. It is capable of separating and analyzing the molecular weight distribution of polymers and macromolecules in solution.

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Lab products found in correlation

Pl gel 5 μm mixed c columns, by Agilent Technologies (9 mentions) Gpc sec software, by Agilent Technologies (4 mentions) Agilent gpc sec software, by Agilent Technologies (1 mentions)

Close spelling variants of this product

1260 Infinity GPC/SEC system Agilent 1260 Infinity II Multi-Detector GPC/SEC System 1260 Infinity II Multi-Detector GPC/SEC System 1260 Infinity II GPC/SEC system Agilent 1260 Infinity Multi-Detector GPC/SEC System SECurity GPC System 1260 Infinity 1260 Infinity II High Temperature GPC System 1260 Infinity Multi-Detector GPC/SEC System 1260 Infinity system 1260 Infinity

9 protocols using 1260 infinity gpc system

Molecular Weight Analysis of Copolymers

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Copolymer molecular
weights and dispersities were determined using an Agilent 1260 Infinity
GPC system equipped with both refractive index and UV–vis detectors.
Two Agilent PL gel 5 μm Mixed-C columns and a guard column were
connected in series and maintained at 60 °C. HPLC-grade DMF containing
10 mM LiBr was used as eluent and the flow rate was set at 1.0 mL
min^–1, with DMSO used as a flow-rate marker. The
refractive index detector was used for calculation of molecular weights
and dispersities by calibration using a series of ten near-monodisperse
poly(methyl methacrylate) standards (with M_n values ranging from 625 to 618 000 g mol^–1; see Figure S4). UV GPC chromatograms
were obtained simultaneously by detection at a fixed wavelength of
309 nm, which corresponds to the absorption maximum for the dithiobenzoate
RAFT end-groups.

Jesson C.P., Cunningham V.J., Smallridge M.J, & Armes S.P. (2018). Synthesis of High Molecular Weight Poly(glycerol monomethacrylate) via RAFT Emulsion Polymerization of Isopropylideneglycerol Methacrylate. Macromolecules, 51(9), 3221-3232.

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Molecular Weight Characterization of (Co)polymers

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DMF gel permeation
chromatography (GPC) was used to assess the molecular weight distribution
of each (co)polymer. The instrument setup comprised two Agilent PL
gel 5 μm Mixed-C columns and a guard column connected in series
to an Agilent 1260 Infinity GPC system operating at 60 °C. The
GPC eluent was high-performance liquid chromatography-grade DMF containing
10 mM LiBr at a flow rate of 1.0 mL min^–1, the copolymer
concentration was typically 1.0% w/w, and calibration was achieved
using a series of ten near-monodisperse poly(methyl methacrylate)
(PMMA) standards ranging from 1080 to 905,000 g mol^–1. Chromatograms were analyzed using Agilent GPC/SEC software.

Astier S., Johnson E.C., Norvilaite O., Varlas S., Brotherton E.E., Sanderson G., Leggett G.J, & Armes S.P. (2024). Controlling Adsorption of Diblock Copolymer Nanoparticles onto an Aldehyde-Functionalized Hydrophilic Polymer Brush via pH Modulation. Langmuir, 40(7), 3667-3676.

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Polymer Molecular Weight Determination

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An Agilent 1260
Infinity GPC system equipped with a differential refractive index
detector and a UV detector was used to determine the number-average
molecular weight (M_n), weight-average
molecular weight (M_w), and dispersity
(M_w/M_n) for
each (co)polymer. Two Agilent PL-gel 5 μm mixed-C columns and
a guard column were connected in series to this GPC system. Unless
otherwise stated, high-performance liquid chromatography (HPLC) grade
DMF containing 10 mM LiBr was used as the eluent. GPC analysis was
performed at 60 °C using a constant flow rate of 1.0 mL min^–1. A series of near-monodisperse poly(methyl methacrylate)
calibration standards with M_p values ranging
from 800 g mol^–1 to 2 200 000 g mol^–1 were used to calculate molecular weights and dispersities.
All (co)polymer samples were diluted to 1.0% w/w using the GPC eluent
and chromatograms were analyzed using Agilent GPC/SEC software.

Hunter S.J., Chohan P., Varlas S, & Armes S.P. (2024). Effect of Temperature, Oil Type, and Copolymer Concentration on the Long-Term Stability of Oil-in-Water Pickering Nanoemulsions Prepared Using Diblock Copolymer Nanoparticles. Langmuir, 40(7), 3702-3714.

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Molecular Weight Characterization by GPC

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DMF GPC was used to determine the number-average molecular weight (M_n) and dispersity (Đ) for all homopolymers and diblock copolymers. The instrument set-up comprised two Agilent PL gel 5 μm Mixed-C columns and a guard column connected in series to an Agilent 1260 Infinity GPC system operating at 60 °C. The GPC eluent was HPLC-grade DMF containing 10 mmol LiBr at a flow rate of 1.0 mL min⁻¹, the copolymer concentration was typically 1.0% w/w, and calibration was achieved using a series of ten near-monodisperse poly(methyl methacrylate) standards ranging from 1080 g mol⁻¹ to 905 000 g mol⁻¹. Chromatograms were analyzed using Agilent GPC/SEC software.

Brotherton E.E., Neal T.J., Kaldybekov D.B., Smallridge M.J., Khutoryanskiy V.V, & Armes S.P. (2022). Aldehyde-functional thermoresponsive diblock copolymer worm gels exhibit strong mucoadhesion. Chemical Science, 13(23), 6888-6898.

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Copolymer Molecular Weight Analysis by DMF GPC

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Copolymer molecular
weight distributions were assessed using DMF GPC. The setup was comprised
of two Agilent PL gel 5 μm Mixed-C columns and a guard column
connected in series to an Agilent 1260 Infinity GPC system equipped
with both refractive index and UV–vis detectors (only the refractive
index detector used) operating at 60 °C. The GPC eluent was HPLC-grade
DMF containing 10 mM LiBr at a flow rate of 1.0 mL min^–1. DMSO was used as a flow-rate marker. Calibration was achieved using
a series of ten near-monodisperse poly(methyl methacrylate) standards
(ranging in M_p from 625 to 618 000
g mol^–1). Chromatograms were analyzed using Agilent
GPC/SEC software.

Byard S.J., Williams M., McKenzie B.E., Blanazs A, & Armes S.P. (2017). Preparation and Cross-Linking of All-Acrylamide Diblock Copolymer Nano-Objects via Polymerization-Induced Self-Assembly in Aqueous Solution. Macromolecules, 50(4), 1482-1493.

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Copolymer Characterization by GPC

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Copolymer molecular
weights and polydispersities were determined using an Agilent 1260
Infinity GPC system equipped with both refractive index and UV–vis
detectors. Two Agilent PL gel 5 μm Mixed-C columns and a guard
column were connected in series and maintained at 60 °C. HPLC-grade
DMF containing 10 mM LiBr was used as eluent, and the flow rate was
set at 1.0 mL min^–1. DMSO was used as a flow-rate
marker. The refractive index detector was used for calculation of
molecular weights and polydispersities by calibration using a series
of ten near-monodisperse poly(methyl methacrylate) standards (with M_n values ranging from 625 to 618 000
g mol^–1). UV GPC chromatograms were obtained simultaneously
by detection at a fixed wavelength of 309 nm which corresponds to
the absorption maximum assigned to the dithiobenzoate or trithiocarbonate
RAFT end-groups.

Jesson C.P., Pearce C.M., Simon H., Werner A., Cunningham V.J., Lovett J.R., Smallridge M.J., Warren N.J, & Armes S.P. (2016). H2O2 Enables Convenient Removal of RAFT End-Groups from Block Copolymer Nano-Objects Prepared via Polymerization-Induced Self-Assembly in Water. Macromolecules, 50(1), 182-191.

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Copolymer Molecular Weight Analysis

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Copolymer molecular weights
and dispersities were determined using an Agilent 1260 Infinity GPC
system equipped with a refractive index detector and a UV–visible
detector. Two Agilent PLgel 5 μm Mixed-C columns and a guard
column were connected in series and maintained at 60 °C. HPLC-grade
DMF containing 10 mM LiBr was used as the eluent, and the flow rate
was set at 1.0 mL min^–1. A refractive index detector
was used to calculate molecular weights and dispersities using a series
of 10 near-monodisperse poly(methyl methacrylate) calibration standards
(with M_n values ranging from 370 to 2,520,000
g mol^–1).

Deane O.J., Mandrelier P., Musa O.M., Jamali M., Fielding L.A, & Armes S.P. (2024). Synthesis and Characterization of All-Acrylic Tetrablock Copolymer Nanoparticles: Waterborne Thermoplastic Elastomers via One-Pot RAFT Aqueous Emulsion Polymerization. Chemistry of Materials, 36(4), 2061-2075.

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Gel Permeation Chromatography for Polymer Analysis

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Number-average molecular
weights (M_n), weight-average molecular
weights (M_w), and dispersities (M_w/M_n) were determined
using an Agilent 1260 Infinity GPC system operating at 60 °C
and equipped with two Agilent PL-gel 5 μm Mixed-C columns, a
guard column, a differential refractive index detector, and a UV–visible
detector operating at 305 nm. Unless otherwise stated, the eluent
was HPLC-grade DMF containing 10 mM LiBr and a flow rate of 1.0 mL
min^–1. For GPC analysis of the PPO-TTC precursors,
LiBr was omitted from the eluent because the presence of this salt
produced a signal that overlaps with the polymer signal (see Figure S3). A series of near-monodisperse poly(methyl
methacrylate) polymers with M_p values
ranging from 800 to 2,200,000 g mol^–1 was used for
calibration. Samples were prepared for GPC analysis by diluting using
the eluent, and chromatograms were analyzed using Agilent GPC/SEC
software.

Farmer M.A., Musa O.M., Haug I., Naumann S, & Armes S.P. (2023). Synthesis of Poly(propylene oxide)–Poly(N,N′-dimethylacrylamide) Diblock Copolymer Nanoparticles via Reverse Sequence Polymerization-Induced Self-Assembly in Aqueous Solution. Macromolecules, 57(1), 317-327.

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DMF GPC for Polymer Characterization

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. DMF GPC was used to determine the number-average molecular weights (M n ) and dispersities (Đ) for all (co)polymers. The instrument set-up comprised two Agilent PL gel 5 μm Mixed-C columns and a guard column connected in series to an Agilent 1260 Infinity GPC system operating at 60 °C. The GPC eluent was HPLC-grade DMF containing 10 mmol LiBr at a flow rate of 1.0 mL min -1 , the copolymer concentration was typically 1.0% w/w and calibration was achieved using a series of ten near-monodisperse poly(methyl methacrylate) standards ranging from 1 080 g mol -1 to 905 000 g mol -1 . Chromatograms were analyzed using Agilent GPC/SEC software.

Brotherton E.E., Smallridge M.J, & Armes S.P. (2021). Aldehyde-Functional Diblock Copolymer Nano-objects via RAFT Aqueous Dispersion Polymerization. Biomacromolecules, 22(12).

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