Jon a antibody

Absolute Zero e‐liquid (18 mg nicotine, 30% propylene glycol, 70% vegetable glycerin with a menthol flavor) was obtained from The Vapor Chef (Bristol, PA). ADP, prostacyclin/prostaglandin I₂ (PGI₂), cotinine methanol solution, and cotinine‐D3 standard were purchased from Sigma Aldrich (St. Louis, MO). U46619 was purchased from Abcam (St. Louis, MO). Fluorescein isothiocyanate (FITC)‐conjugated anti‐P‐selectin and FITC‐conjugated Annexin V were purchased from Cell Signaling Technology, Inc (Danvers, MA). The JON/A antibody was obtained from Emfret analytics (Würzburg, Germany). The phycoerythrin‐conjugated anti‐CD69 and FITC‐conjugated anti‐CD45 were obtained from BD Biosciences (San Jose, CA). Stir bars and other disposables were purchased from Chrono‐Log Corporation (Havertown, PA). Kinetex 1.7 μm EVO C18 100 A‐LC Column 100×2.1 mm, Security Guard ULTRA holder for UHPLC Columns 2.1 to 6.4 mm, and SecurityGuard ULTRA cartridges for EVO‐C18‐UHPLC were purchased from Phenomenex Inc (Torrance, CA). Other reagents were of analytical grade.

JUUL device and JUUL Pods (5% nicotine and menthol flavoring) were purchased from the JUUL official website. Absolute 0 e-liquid (18-mg Nicotine, 30% PG, and 70% VG with a menthol flavor) was obtained from The Vapor Chef. Adenosine diphosphate (ADP) and thrombin were purchased from Sigma Aldrich. The anti-P-selectin and Annexin V antibodies were fluorescein isothiocyanate (FITC)–conjugated and purchased from Cell Signaling Technology, Inc. The JON/A antibody was purchased from Emfret analytics. The Mouse/Rat Cotinine enzyme-linked immunosorbent assay (ELISA) kit was from Calbiotech. The stir bars and other experimental disposable material was obtained from Chrono-Log Corporation. The rest of the reagents were of analytical grade.