Onetouch ultra blood glucose meter and test strips

The glucose tolerance test (GTT) was carried out on 8–10-week-old male mice unless otherwise indicated. Prior to studies, mice were fasted overnight (GTT). In GTT studies, the mice were injected intraperitoneally (IP) with d-glucose solution (2 g/kg body weight). The tail blood glucose concentration was measured at 0, 15, 30, 60 and 120 min with a commercially available ONETOUCH Ultra blood glucose meter and test strips (Life Scan). A hyperglycemicclamp was performed, and calculated as described previously.^{33 (link)}

Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured using commercial kits (Diagnostic Chemicals, Ltd., Oxford, CT). Plasma and hepatic triglyceride levels were determined by using Triglyceride GPO reagent (Pointe Scientific, Lincoln Park, MI). Hepatic and plasma total cholesterol, plasma HDLc (in ApoB-depleted plasma), and hepatic total palmitate levels were measured by GC-MS [19 (link)]. Blood glucose was monitored using the OneTouch Ultra Blood Glucose Meter and test strips (Lifescan, Milpitas, CA) and plasma insulin levels were measured using an ELISA assay (Mercodia, Uppsala, Sweden).